ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSDAEEEVVE YEEEQEEQEE AVEEEDGEAE PDPEGEAEAE EDKAEEVGPD EEARDAEDGP 

        70         80         90        100        110        120 
VEDSKPKPSR LFMPNLVPPK IPDGERVDFD DIHRKRMEKD LNELQTLIEA HFENRKKEEE 

       130        140        150        160        170        180 
ELISLKDRIE KRRAERAEQQ RIRNEREKER QNRLAEERAR REEEENRRKA EDEARKKKAL 

       190        200        210        220        230        240 
SNMMHFGGYI QKAQTERKSG KRQTEREKKK KILAERRKVL AIDHLNEDQL REKAKELWQS 

       250        260        270        280 
IHNLEAEKFD LQEKFKQQKY EINVLRNRIN DNQKVSKTRG KAKVTGRWK 

References and documentation are available.

Number of amino acids: 289

Molecular weight: 34452.12

Theoretical pI: 5.19

Amino acid composition: 
Ala (A)  22	  7.6%
Arg (R)  31	 10.7%
Asn (N)  13	  4.5%
Asp (D)  19	  6.6%
Cys (C)   0	  0.0%
Gln (Q)  15	  5.2%
Glu (E)  61	 21.1%
Gly (G)  10	  3.5%
His (H)   5	  1.7%
Ile (I)  12	  4.2%
Leu (L)  17	  5.9%
Lys (K)  34	 11.8%
Met (M)   5	  1.7%
Phe (F)   6	  2.1%
Pro (P)  10	  3.5%
Ser (S)   8	  2.8%
Thr (T)   5	  1.7%
Trp (W)   2	  0.7%
Tyr (Y)   3	  1.0%
Val (V)  11	  3.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 80
Total number of positively charged residues (Arg + Lys): 65

Atomic composition:

Carbon      C	      1465
Hydrogen    H	      2386
Nitrogen    N	       456
Oxygen      O	       494
Sulfur      S	         5

Formula: C1465H2386N456O494S5
Total number of atoms: 4806

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    15470
Abs 0.1% (=1 g/l)   0.449

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 71.02
This classifies the protein as unstable.



Aliphatic index: 57.79

Grand average of hydropathicity (GRAVY): -1.630