ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSDAGKKISR KEAKKAKAKE NYDKTILSMG GVVENLPEVE GDQEEHGGIG SGAELGAHFT 

        70         80         90        100        110        120 
VSQLSKTGTQ LAQMENSMDI KIENFDISAQ GKLLFDKASL TIVYGRRYGL VGPNGMGKTT 

       130        140        150        160        170        180 
LLKHIGARKL AIPSHIDLLY CEQEIQVDST SAIDTVVKSD KKRLALLEEE AKLMSEIEEG 

       190        200        210        220        230        240 
KTEAAERMKE VADELRDIGA DSAEPRARRI LAGLGFSKEM QEKPCTDFSG GWRMRISLAR 

       250        260        270        280        290        300 
ALFLEPTLLM LDEPTNHLDL NAVIWLDNYL QTWKKTLLIV SHDQGFLDSV CTDIIHLDNQ 

       310        320        330        340        350        360 
KLHTYRGNYT LFKKQYAQDM QVHEKNFDQQ QKQLKAMKKE GKSAKQAEEQ VKQQMANKAK 

       370        380        390        400        410        420 
KGGKKNAGKV NDDDDAGAPE LLQRRKEYSV KFQFPETTKL NPPVLGLHDV NFGYGKDVLF 

       430        440        450        460        470        480 
KKLNFGVDMD SRIAIVGPNG VGKSTLLKLL IGKIDPNDGE LRKHRTLRIG WFDQHANEAL 

       490        500        510        520        530        540 
NGEQTPVEFL CTKFNIDYQE ARKQLGTTGL AAHAHTVKIK DLSGGQKSRV ALCNLALGGP 

       550        560        570        580        590        600 
DIIILDEPTN NLDIESIDAL AEAIRDFNGG VVMVTHDERL VVRTDCNLWV VENQGIDEID 

       610        620 
GDFEDYKKEV LDALGEALVS KK 

References and documentation are available.

Number of amino acids: 622

Molecular weight: 69175.83

Theoretical pI: 5.93

Amino acid composition: 
Ala (A)  47	  7.6%
Arg (R)  25	  4.0%
Asn (N)  28	  4.5%
Asp (D)  48	  7.7%
Cys (C)   6	  1.0%
Gln (Q)  30	  4.8%
Glu (E)  48	  7.7%
Gly (G)  52	  8.4%
His (H)  15	  2.4%
Ile (I)  35	  5.6%
Leu (L)  67	 10.8%
Lys (K)  61	  9.8%
Met (M)  15	  2.4%
Phe (F)  19	  3.1%
Pro (P)  16	  2.6%
Ser (S)  28	  4.5%
Thr (T)  30	  4.8%
Trp (W)   5	  0.8%
Tyr (Y)  12	  1.9%
Val (V)  35	  5.6%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 96
Total number of positively charged residues (Arg + Lys): 86

Atomic composition:

Carbon      C	      3043
Hydrogen    H	      4907
Nitrogen    N	       851
Oxygen      O	       943
Sulfur      S	        21

Formula: C3043H4907N851O943S21
Total number of atoms: 9765

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    45755
Abs 0.1% (=1 g/l)   0.661, assuming all pairs of Cys residues form cystines


Ext. coefficient    45380
Abs 0.1% (=1 g/l)   0.656, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 28.55
This classifies the protein as stable.



Aliphatic index: 87.83

Grand average of hydropathicity (GRAVY): -0.493