ProtParam
User-provided sequence:
10 20 30 40 50 60
MSDAQFDAAL DLLRRLNPTT LQENLNNLIE LQPNLAQDLL SSVDVPLSTQ KDSADSNREY
70 80 90 100 110 120
LCCDYNRDID SFRSPWSNTY YPELSPKDLQ DSPFPSAPLR KLEILANDSF DVYRDLYYEG
130 140 150 160 170 180
GISSVYLWDL NEEDFNGHDF AGVVLFKKNQ SDHSNWDSIH VFEVTTSPSS PDSFNYRVTT
190 200 210 220 230 240
TIILHLDKTK TDQNSHMMLS GNLTRQTEKD IAIDMSRPLD VIFTSHVANL GSLIEDIESQ
250 260 270 280
MRNLLETVYF EKTRDIFHQT KNAAIASSAE EANKDAQAEV IRGLQSL
References and
documentation are available.
Number of amino acids: 287
Molecular weight: 32629.11
Theoretical pI: 4.52
Total number of negatively charged residues (Asp + Glu): 46
Total number of positively charged residues (Arg + Lys): 24
Atomic composition:
Carbon C 1433
Hydrogen H 2219
Nitrogen N 387
Oxygen O 471
Sulfur S 7
Formula: C
1433H
2219N
387O
471S
7
Total number of atoms: 4517
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 31525
Abs 0.1% (=1 g/l) 0.966, assuming all pairs of Cys residues form cystines
Ext. coefficient 31400
Abs 0.1% (=1 g/l) 0.962, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 58.90
This classifies the protein as unstable.
Aliphatic index: 84.29
Grand average of hydropathicity (GRAVY): -0.517