ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSDDHGYERD NHLSRRSGNY NGRRKFADTY RGSRDRGEYR GGRERSDYRE RERFNNRDNP 

        70         80         90        100        110        120 
RSRDRYDDRR RGRDVTGRYG NRRDDYPRSF RSRHNTRDDS RRGGFGSSGA RGDYGPLLAR 

       130        140        150        160        170        180 
ELDSTYEEKV NRNYSNSIFV GNLTYDSTPE DLTEFFSQIG KVVRADIITS RGHHRGMGTV 

       190        200        210        220        230        240 
EFTNSDDVDR AIRQYDGAFF MDRKIFVRQD NPPPSNNIKE RKALDRGELR HNRKTHEVIV 

       250        260        270        280        290        300 
KNLPASVNWQ ALKDIFKECG NVAHADVELD GDGVSTGSGT VSFYDIKDLH RAIEKYNGYS 

       310        320        330        340        350        360 
IEGNVLDVKS KESVHNHSDG DDVDIPMDDS PVNEEARKFT ENVVGGGERN RLIYCSNLPF 

       370        380        390        400        410        420 
STAKSDLYDL FETIGKVNNA ELRYDSKGAP TGIAVVEYDN VDDADVCIER LNNYNYGGCD 


LDISYAKRL 

References and documentation are available.

Number of amino acids: 429

Molecular weight: 49141.53

Theoretical pI: 6.18

Amino acid composition: 
Ala (A)  19	  4.4%
Arg (R)  52	 12.1%
Asn (N)  33	  7.7%
Asp (D)  49	 11.4%
Cys (C)   4	  0.9%
Gln (Q)   4	  0.9%
Glu (E)  27	  6.3%
Gly (G)  39	  9.1%
His (H)  11	  2.6%
Ile (I)  18	  4.2%
Leu (L)  21	  4.9%
Lys (K)  19	  4.4%
Met (M)   4	  0.9%
Phe (F)  16	  3.7%
Pro (P)  12	  2.8%
Ser (S)  33	  7.7%
Thr (T)  17	  4.0%
Trp (W)   1	  0.2%
Tyr (Y)  23	  5.4%
Val (V)  27	  6.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 76
Total number of positively charged residues (Arg + Lys): 71

Atomic composition:

Carbon      C	      2100
Hydrogen    H	      3264
Nitrogen    N	       664
Oxygen      O	       692
Sulfur      S	         8

Formula: C2100H3264N664O692S8
Total number of atoms: 6728

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    40020
Abs 0.1% (=1 g/l)   0.814, assuming all pairs of Cys residues form cystines


Ext. coefficient    39770
Abs 0.1% (=1 g/l)   0.809, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 39.89
This classifies the protein as stable.



Aliphatic index: 58.14

Grand average of hydropathicity (GRAVY): -1.100