ProtParam
User-provided sequence:
10 20 30 40 50 60
MSDDLRRKIA LSQFERAKNV LDATFQEAYE DDENDGDALG SLPSFNGQSN RNRKYTGKTG
70 80 90 100 110 120
STTDRISSKE KSSLPTWSDF FDNKELVSLP DRDLDVNTYY TLPTSLLSNT TSIPIFIFHH
130 140 150 160 170 180
GAGSSGLSFA NLAKELNTKL EGRCGCFAFD ARGHAETKFK KADAPICFDR DSFIKDFVSL
190 200 210 220 230 240
LNYWFKSKIS QEPLQKVSVI LIGHSLGGSI CTFAYPKLST ELQKKILGIT MLDIVEEAAI
250 260 270 280 290 300
MALNKVEHFL QNTPNVFESI NDAVDWHVQH ALSRLRSSAE IAIPALFAPL KSGKVVRITN
310 320 330 340 350 360
LKTFSPFWDT WFTDLSHSFV GLPVSKLLIL AGNENLDKEL IVGQMQGKYQ LVVFQDSGHF
370 380 390 400
IQEDSPIKTA ITLIDFWKRN DSRNVVIKTN WGQHKTVQNT
References and
documentation are available.
Number of amino acids: 400
Molecular weight: 44887.94
Theoretical pI: 6.56
Total number of negatively charged residues (Asp + Glu): 47
Total number of positively charged residues (Arg + Lys): 45
Atomic composition:
Carbon C 2020
Hydrogen H 3147
Nitrogen N 539
Oxygen O 603
Sulfur S 8
Formula: C
2020H
3147N
539O
603S
8
Total number of atoms: 6317
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 49180
Abs 0.1% (=1 g/l) 1.096, assuming all pairs of Cys residues form cystines
Ext. coefficient 48930
Abs 0.1% (=1 g/l) 1.090, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 31.67
This classifies the protein as stable.
Aliphatic index: 86.80
Grand average of hydropathicity (GRAVY): -0.273