ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSDDRFAEDE IIQQRRKRRL EILKKYQQTG NGHSDLSIPE KKLKEDVDQV STTKPIEAVP 

        70         80         90        100        110        120 
KMKTNASKIE INKEGSNSNT KLDVTNSTTS DSPSIKSSVQ IEDTEDDMFA DSPSPSVKRQ 

       130        140        150        160        170        180 
NTGKGISTLT RSFADMQDNW DDIEGYYKVV LMEELDSRYI VQSNLGKGMF STVVSALDRN 

       190        200        210        220        230        240 
RNQTFAIKII RNNEVMYKEG LKEVSILERL QAADREGKQH IIHYERHFMH KNHLCMVFEM 

       250        260        270        280        290        300 
LSLNLRDILK KFGRNVGLSI KAVRLYAYQM FMALDLLKQC NVIHSDIKPD NMLVNEKRNI 

       310        320        330        340        350        360 
LKICDLGSAS DASENEITPY LVSRFYRAPE IILGFPYSCP IDTWSVGCSL YELYTGQILF 

       370        380        390        400        410        420 
PGRTNNQMLR YMMECKGKFS HKMLKRSQFL NDHFDADFNF IQIDHDPITN QETRKPVKFS 

       430        440        450        460        470 
KPTKDIRSRL KEVPTSTDEE FIIRQELMDL LEKCLELNPE KRVPPEVALK HPFFIKK 

References and documentation are available.

Number of amino acids: 477

Molecular weight: 55222.27

Theoretical pI: 8.00

Amino acid composition: 
Ala (A)  17	  3.6%
Arg (R)  28	  5.9%
Asn (N)  27	  5.7%
Asp (D)  34	  7.1%
Cys (C)   7	  1.5%
Gln (Q)  20	  4.2%
Glu (E)  35	  7.3%
Gly (G)  18	  3.8%
His (H)  11	  2.3%
Ile (I)  36	  7.5%
Leu (L)  41	  8.6%
Lys (K)  43	  9.0%
Met (M)  18	  3.8%
Phe (F)  21	  4.4%
Pro (P)  20	  4.2%
Ser (S)  38	  8.0%
Thr (T)  23	  4.8%
Trp (W)   2	  0.4%
Tyr (Y)  14	  2.9%
Val (V)  24	  5.0%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 69
Total number of positively charged residues (Arg + Lys): 71

Atomic composition:

Carbon      C	      2434
Hydrogen    H	      3893
Nitrogen    N	       675
Oxygen      O	       738
Sulfur      S	        25

Formula: C2434H3893N675O738S25
Total number of atoms: 7765

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    32235
Abs 0.1% (=1 g/l)   0.584, assuming all pairs of Cys residues form cystines


Ext. coefficient    31860
Abs 0.1% (=1 g/l)   0.577, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 50.58
This classifies the protein as unstable.



Aliphatic index: 81.11

Grand average of hydropathicity (GRAVY): -0.589