ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSDDRINYLK NKLVQYPDFP KKGILFEDIM PIFQDPRAFG ILIDLLLEAV ETEFNNIDVI 

        70         80         90        100        110        120 
VGLEARGFLF GPTLALRANC AFVPVRKPNK LPGDLVVVSY NKEYSTDSFA IQKGTIKPGQ 

       130        140        150        160        170        180 
RVLIVDDILA TGGTALAADE LVTRLGGELV GHLFLLELTF LQGRKRLMAP TYTLLTGQDE 


APDGSEFA 

References and documentation are available.

Number of amino acids: 188

Molecular weight: 20746.03

Theoretical pI: 4.85

Amino acid composition: 
Ala (A)  14	  7.4%
Arg (R)   9	  4.8%
Asn (N)   7	  3.7%
Asp (D)  14	  7.4%
Cys (C)   1	  0.5%
Gln (Q)   6	  3.2%
Glu (E)  11	  5.9%
Gly (G)  16	  8.5%
His (H)   1	  0.5%
Ile (I)  12	  6.4%
Leu (L)  27	 14.4%
Lys (K)  10	  5.3%
Met (M)   3	  1.6%
Phe (F)  12	  6.4%
Pro (P)  11	  5.9%
Ser (S)   5	  2.7%
Thr (T)  11	  5.9%
Trp (W)   0	  0.0%
Tyr (Y)   5	  2.7%
Val (V)  13	  6.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 25
Total number of positively charged residues (Arg + Lys): 19

Atomic composition:

Carbon      C	       947
Hydrogen    H	      1502
Nitrogen    N	       240
Oxygen      O	       273
Sulfur      S	         4

Formula: C947H1502N240O273S4
Total number of atoms: 2966

Extinction coefficients:

This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient     7450
Abs 0.1% (=1 g/l)   0.359, assuming all pairs of Cys residues form cystines


Ext. coefficient     7450
Abs 0.1% (=1 g/l)   0.359, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 30.28
This classifies the protein as stable.



Aliphatic index: 108.40

Grand average of hydropathicity (GRAVY): 0.108