ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSDDSSSPLT TLKILIIGES GVGKSSLMLR FVDDVFDPEQ AATIGVDFRV TSMAIDGNRV 

        70         80         90        100        110        120 
KLAIWDTAGQ ERFRTLTPSY YRGAQGVICV YDVTSRSSFE KLNHWMQEVD TYCTNDNIIK 

       130        140        150        160        170        180 
MMVANKIDMP NRVVTREEGL KFAKRHRTLF IEASAKTKEG VQCTFEELIE KIIQTPDLWD 

       190        200 
NDRPSFRLGQ PTGSSGGGGM CGC 

References and documentation are available.

Number of amino acids: 203

Molecular weight: 22619.75

Theoretical pI: 5.49

Amino acid composition: 
Ala (A)  10	  4.9%
Arg (R)  13	  6.4%
Asn (N)   7	  3.4%
Asp (D)  15	  7.4%
Cys (C)   5	  2.5%
Gln (Q)   7	  3.4%
Glu (E)  12	  5.9%
Gly (G)  17	  8.4%
His (H)   2	  1.0%
Ile (I)  14	  6.9%
Leu (L)  13	  6.4%
Lys (K)  11	  5.4%
Met (M)   8	  3.9%
Phe (F)   9	  4.4%
Pro (P)   7	  3.4%
Ser (S)  16	  7.9%
Thr (T)  16	  7.9%
Trp (W)   3	  1.5%
Tyr (Y)   4	  2.0%
Val (V)  14	  6.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 27
Total number of positively charged residues (Arg + Lys): 24

Atomic composition:

Carbon      C	       987
Hydrogen    H	      1570
Nitrogen    N	       274
Oxygen      O	       308
Sulfur      S	        13

Formula: C987H1570N274O308S13
Total number of atoms: 3152

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    22710
Abs 0.1% (=1 g/l)   1.004, assuming all pairs of Cys residues form cystines


Ext. coefficient    22460
Abs 0.1% (=1 g/l)   0.993, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 45.81
This classifies the protein as unstable.



Aliphatic index: 76.80

Grand average of hydropathicity (GRAVY): -0.291