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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSDEAKEKRE LESQKESSHN KSEKSVEPKP KRRRRRNYDD YDAEVAKEET KAKNGLTKSE

70 80 90 100 110 120
NNGTVEDSES DMDDAKLDAL MGNEGEEEED DLAEIDTSNI ITSGRRTRGK VIDYKKTAEE

130 140 150
LDKKEPSTGS KDDVGYGEKE EDDEDEEDDD FKE


References and documentation are available.

Number of amino acids: 153 Molecular weight: 17462.50 Theoretical pI: 4.44
Amino acid composition: 
Ala (A) 8 5.2% Arg (R) 9 5.9% Asn (N) 7 4.6% Asp (D) 22 14.4% Cys (C) 0 0.0% Gln (Q) 1 0.7% Glu (E) 29 19.0% Gly (G) 9 5.9% His (H) 1 0.7% Ile (I) 4 2.6% Leu (L) 6 3.9% Lys (K) 20 13.1% Met (M) 3 2.0% Phe (F) 1 0.7% Pro (P) 3 2.0% Ser (S) 13 8.5% Thr (T) 8 5.2% Trp (W) 0 0.0% Tyr (Y) 4 2.6% Val (V) 5 3.3% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 51 Total number of positively charged residues (Arg + Lys): 29 Atomic composition: Carbon C 719 Hydrogen H 1156 Nitrogen N 210 Oxygen O 289 Sulfur S 3 Formula: C719H1156N210O289S3 Total number of atoms: 2377 Extinction coefficients: This protein does not contain any Trp residues. Experience shows that this could result in more than 10% error in the computed extinction coefficient. Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 5960 Abs 0.1% (=1 g/l) 0.341 Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 71.23 This classifies the protein as unstable. Aliphatic index: 40.20 Grand average of hydropathicity (GRAVY): -1.785