ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSDEDSMLLN FTTNEDTAGS SYKQAAKVTG GRWKDRRRMK MKLEGKTVSR KRKANTTGDE 

        70         80         90        100        110        120 
GIIPGRGENS IKKLHKESSY SSEEQEKYKG RNAHNTQGRT LPADSQFVSS LFTSNREITT 

       130        140        150        160        170        180 
AVNTNIHDEN VAINPSNAPL KGDQFASLGV SSLLVSHLEQ KMRIKKPTSI QKQAIPQIIG 

       190        200        210        220        230        240 
NAGKNDFFIH AQTGSGKTLS YLLPIISTIL NMDTHVDRTS GAFALVIAPT RELASQIYHV 

       250        260        270        280        290        300 
CSTLVSCCHY LVPCLLIGGE RKKSEKARLR KGCNFIIGTP GRVLDHLQNT KVIKEQLSQS 

       310        320        330        340        350        360 
LRYIVLDEGD KLMELGFDET ISEIIKIVHD IPINSEKFPK LPHKLVHMLC SATLTDGVNR 

       370        380        390        400        410        420 
LRNVALKDYK LISNGTKKDS DIVTVAPDQL LQRITIVPPK LRLVTLAATL NNITKDFIAS 

       430        440        450        460        470        480 
GQQSKTLRTI VFVSCSDSVE FHYDAFSGSD GHHKNLTGDS VRLLTKGNTM FPCFSDSRDP 

       490        500        510        520        530        540 
DVVIYKLHGS LSQQMRTSTL QHFARDNEAT KGKHLIMFCT DVASRGLDLP HVGSVIELDP 

       550        560        570        580        590        600 
PFAVEDHLHR VGRTARAGEK GESLLFLLPG EEEKYMDYIQ PYHPMGWELL KFDKEILMPA 

       610        620        630        640        650        660 
FKDVNVNRND KFIRKDEKSS KNKDVGDKEY EWDTNATTWH LNIERRVVGD SAFKNLAVKG 

       670        680        690        700        710        720 
FISHVRAYAT HISQEKKFFN VKFLHLGHLA KSFGLRERPK AMGLQSSKDG NSEKKPTKEN 

       730        740 
SKNKMFRMAR MAEKQIASEF NY 

References and documentation are available.

Number of amino acids: 742

Molecular weight: 83308.25

Theoretical pI: 9.33

Amino acid composition: 
Ala (A)  42	  5.7%
Arg (R)  40	  5.4%
Asn (N)  38	  5.1%
Asp (D)  44	  5.9%
Cys (C)   9	  1.2%
Gln (Q)  25	  3.4%
Glu (E)  42	  5.7%
Gly (G)  47	  6.3%
His (H)  27	  3.6%
Ile (I)  45	  6.1%
Leu (L)  68	  9.2%
Lys (K)  68	  9.2%
Met (M)  18	  2.4%
Phe (F)  31	  4.2%
Pro (P)  27	  3.6%
Ser (S)  62	  8.4%
Thr (T)  47	  6.3%
Trp (W)   4	  0.5%
Tyr (Y)  16	  2.2%
Val (V)  42	  5.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 86
Total number of positively charged residues (Arg + Lys): 108

Atomic composition:

Carbon      C	      3674
Hydrogen    H	      5899
Nitrogen    N	      1051
Oxygen      O	      1103
Sulfur      S	        27

Formula: C3674H5899N1051O1103S27
Total number of atoms: 11754

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    46340
Abs 0.1% (=1 g/l)   0.556, assuming all pairs of Cys residues form cystines


Ext. coefficient    45840
Abs 0.1% (=1 g/l)   0.550, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 39.90
This classifies the protein as stable.



Aliphatic index: 81.47

Grand average of hydropathicity (GRAVY): -0.493