ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSDEEEVYSD EEVEEEEVEE TEEAPAEAEE PEETTEEVVA PPEVKERRAP VQEEKPPAEM 

        70         80         90        100        110        120 
TEAEIAMLAA KKRHEEEEAA KLLDYEQRRV LEKEQIEQEL RELKEKQEKR RAEREEDERQ 

       130        140        150        160        170        180 
FAERRRQDDE RRRKEEDERK AKADAEKIRK NEEKVRRQQM MAGAFNGRVE AGAGRNFTVA 

       190        200        210        220        230        240 
SKGDQAAQFG NLAQAKGGKD VMSKEQMEDA KRNFLAAVCR VQDVSGLLPN DLKERIRGLH 

       250        260        270        280        290        300 
ARIVKLEAEK YDLEKRRERQ DYDMKELHER QRQAARNKAL KKGLDPEEAA SSVHPPKITT 

       310        320        330        340        350        360 
ASKFDRQTDR RSYGDRRYLF ENPEEEKQAS LVRGTGRPPA EWGRKQNEEL EQIRKNLEPP 

       370        380        390        400 
KYVEQVKVEG ARAPVEPVPL FVPTDDFEPQ QVTEDPNVGE EVVVE 

References and documentation are available.

Number of amino acids: 405

Molecular weight: 47041.09

Theoretical pI: 4.96

Amino acid composition: 
Ala (A)  41	 10.1%
Arg (R)  43	 10.6%
Asn (N)  11	  2.7%
Asp (D)  23	  5.7%
Cys (C)   1	  0.2%
Gln (Q)  24	  5.9%
Glu (E)  80	 19.8%
Gly (G)  17	  4.2%
His (H)   4	  1.0%
Ile (I)   7	  1.7%
Leu (L)  22	  5.4%
Lys (K)  35	  8.6%
Met (M)   8	  2.0%
Phe (F)   9	  2.2%
Pro (P)  22	  5.4%
Ser (S)  10	  2.5%
Thr (T)  11	  2.7%
Trp (W)   1	  0.2%
Tyr (Y)   7	  1.7%
Val (V)  29	  7.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 103
Total number of positively charged residues (Arg + Lys): 78

Atomic composition:

Carbon      C	      2006
Hydrogen    H	      3247
Nitrogen    N	       613
Oxygen      O	       675
Sulfur      S	         9

Formula: C2006H3247N613O675S9
Total number of atoms: 6550

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    15930
Abs 0.1% (=1 g/l)   0.339, assuming all pairs of Cys residues form cystines


Ext. coefficient    15930
Abs 0.1% (=1 g/l)   0.339, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 75.66
This classifies the protein as unstable.



Aliphatic index: 58.81

Grand average of hydropathicity (GRAVY): -1.333