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ProtParam

User-provided sequence:

        10         20         30         40         50 
MSDEETEQVE EQYEEEEEAQ EEEEVQEDAV AEEEREEDEE EEKPRPKLTA P


References and documentation are available.

Number of amino acids: 51 Molecular weight: 6113.18 Theoretical pI: 3.68
Amino acid composition: 
Ala (A) 4 7.8% Arg (R) 2 3.9% Asn (N) 0 0.0% Asp (D) 3 5.9% Cys (C) 0 0.0% Gln (Q) 4 7.8% Glu (E) 24 47.1% Gly (G) 0 0.0% His (H) 0 0.0% Ile (I) 0 0.0% Leu (L) 1 2.0% Lys (K) 2 3.9% Met (M) 1 2.0% Phe (F) 0 0.0% Pro (P) 3 5.9% Ser (S) 1 2.0% Thr (T) 2 3.9% Trp (W) 0 0.0% Tyr (Y) 1 2.0% Val (V) 3 5.9% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 27 Total number of positively charged residues (Arg + Lys): 4 Atomic composition: Carbon C 249 Hydrogen H 381 Nitrogen N 63 Oxygen O 114 Sulfur S 1 Formula: C249H381N63O114S1 Total number of atoms: 808 Extinction coefficients: This protein does not contain any Trp residues. Experience shows that this could result in more than 10% error in the computed extinction coefficient. Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 1490 Abs 0.1% (=1 g/l) 0.244 Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 139.23 This classifies the protein as unstable. Aliphatic index: 32.55 Grand average of hydropathicity (GRAVY): -2.120