ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSDEETEQVE EQYEEEEEAQ EEEVQEEAPE PEEVQEEEKP RPKLTAPKIP EGEKVDFDDI 

        70         80         90        100        110        120 
QKKRQNKDLM ELQALIDSHF EARKKEEEEL IALKERIEKR RAERAEQQRI RAEKERERQN 

       130        140        150        160        170        180 
RLAEEKARRE EEDAKRRAED DLKKKKALSS MGANYSSYLA KADQKRGKKQ TAREMKKKIL 

       190        200        210        220        230        240 
AERRKPLNID HLSDDKLRDK AKELWDTLYQ LETDKFEFGE KLKRQKYDIT TLRSRIDQAQ 

       250 
KHSKKAGATA KGKVGGRWK 

References and documentation are available.

Number of amino acids: 259

Molecular weight: 30750.43

Theoretical pI: 6.19

Amino acid composition: 
Ala (A)  24	  9.3%
Arg (R)  25	  9.7%
Asn (N)   4	  1.5%
Asp (D)  18	  6.9%
Cys (C)   0	  0.0%
Gln (Q)  17	  6.6%
Glu (E)  48	 18.5%
Gly (G)   8	  3.1%
His (H)   3	  1.2%
Ile (I)  10	  3.9%
Leu (L)  19	  7.3%
Lys (K)  39	 15.1%
Met (M)   4	  1.5%
Phe (F)   4	  1.5%
Pro (P)   7	  2.7%
Ser (S)   9	  3.5%
Thr (T)   8	  3.1%
Trp (W)   2	  0.8%
Tyr (Y)   5	  1.9%
Val (V)   5	  1.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 66
Total number of positively charged residues (Arg + Lys): 64

Atomic composition:

Carbon      C	      1319
Hydrogen    H	      2172
Nitrogen    N	       402
Oxygen      O	       435
Sulfur      S	         4

Formula: C1319H2172N402O435S4
Total number of atoms: 4332

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    18450
Abs 0.1% (=1 g/l)   0.600

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 71.31
This classifies the protein as unstable.



Aliphatic index: 58.53

Grand average of hydropathicity (GRAVY): -1.598