ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSDHEVTGGV SLKERVAIVL VEPQSPGNVG MVCRAMKNMG LGELRIVKGC RLDHPEALKF 

        70         80         90        100        110        120 
AVSARDLLER ARVFESLEDA LSDTELSVAT TRRHGKYRQE IFSPKEIVSK FAANLAGNRA 

       130        140        150        160        170        180 
ALVFGREDNG LTTDELSLCR WHATIPSSEE YGSLNLAQAV MVFCYELFTS LGSRTVGGED 

       190        200        210        220        230        240 
RPLAGSAETE PLFEHMERTL MRIGYLNPQN PAHLMRSLRR IFARSELDSR EVAILRGMMT 

       250 
QIDWAADQFK GKKGA 

References and documentation are available.

Number of amino acids: 255

Molecular weight: 28314.42

Theoretical pI: 6.62

Amino acid composition: 
Ala (A)  24	  9.4%
Arg (R)  23	  9.0%
Asn (N)   8	  3.1%
Asp (D)  11	  4.3%
Cys (C)   4	  1.6%
Gln (Q)   6	  2.4%
Glu (E)  23	  9.0%
Gly (G)  20	  7.8%
His (H)   6	  2.4%
Ile (I)   9	  3.5%
Leu (L)  28	 11.0%
Lys (K)  10	  3.9%
Met (M)  10	  3.9%
Phe (F)  10	  3.9%
Pro (P)   9	  3.5%
Ser (S)  19	  7.5%
Thr (T)  12	  4.7%
Trp (W)   2	  0.8%
Tyr (Y)   4	  1.6%
Val (V)  17	  6.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 34
Total number of positively charged residues (Arg + Lys): 33

Atomic composition:

Carbon      C	      1234
Hydrogen    H	      1990
Nitrogen    N	       362
Oxygen      O	       373
Sulfur      S	        14

Formula: C1234H1990N362O373S14
Total number of atoms: 3973

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    17210
Abs 0.1% (=1 g/l)   0.608, assuming all pairs of Cys residues form cystines


Ext. coefficient    16960
Abs 0.1% (=1 g/l)   0.599, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 37.84
This classifies the protein as stable.



Aliphatic index: 85.33

Grand average of hydropathicity (GRAVY): -0.252