ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSDIDDEEFE QLAVSALEEV EKKAGFAQQP TPQKVSRVTA HSILVNPRQK GNPLLPHVRN 

        70         80         90        100        110        120 
VPWEYTDIVP DFVMGTGICS LFLSLKYHHL HPEYIYSRIS KLGKSYNLRI LLILVDVENH 

       130        140        150        160        170        180 
QASIQELVKT SIVNQYTLIL AWSSEEAARY LETYKAYENM SPALIMEKPS TDYLSQVQSF 

       190        200        210        220        230        240 
LTSIRGINKS DSLSLLSKFG SLERALVASR DELEQLEGWG PTKVNRFLEA VQQPFMSHST 

       250 
IKRPEAINLK QT 

References and documentation are available.

Number of amino acids: 252

Molecular weight: 28637.73

Theoretical pI: 6.00

Amino acid composition: 
Ala (A)  15	  6.0%
Arg (R)  11	  4.4%
Asn (N)  10	  4.0%
Asp (D)   9	  3.6%
Cys (C)   1	  0.4%
Gln (Q)  14	  5.6%
Glu (E)  21	  8.3%
Gly (G)   9	  3.6%
His (H)   7	  2.8%
Ile (I)  16	  6.3%
Leu (L)  30	 11.9%
Lys (K)  15	  6.0%
Met (M)   5	  2.0%
Phe (F)   8	  3.2%
Pro (P)  13	  5.2%
Ser (S)  26	 10.3%
Thr (T)  12	  4.8%
Trp (W)   3	  1.2%
Tyr (Y)  10	  4.0%
Val (V)  17	  6.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 30
Total number of positively charged residues (Arg + Lys): 26

Atomic composition:

Carbon      C	      1287
Hydrogen    H	      2035
Nitrogen    N	       341
Oxygen      O	       385
Sulfur      S	         6

Formula: C1287H2035N341O385S6
Total number of atoms: 4054

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    31400
Abs 0.1% (=1 g/l)   1.096, assuming all pairs of Cys residues form cystines


Ext. coefficient    31400
Abs 0.1% (=1 g/l)   1.096, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 38.97
This classifies the protein as stable.



Aliphatic index: 96.71

Grand average of hydropathicity (GRAVY): -0.277