ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSDIEEGTPT NNGQQKERRK IEIKFIENKT RRHVTFSKRK HGIMKKAFEL SVLTGTQVLL 

        70         80         90        100        110        120 
LVVSETGLVY TFSTPKFEPI VTQQEGRNLI QACLNAPDDE EEDEEEDGDD DDDDDDDGND 

       130        140        150        160        170        180 
MQRQQPQQQQ PQQQQQVLNA HANSLGHLNQ DQVPAGALKQ EVKSQLLGGA NPNQNSMIQQ 

       190        200        210        220        230        240 
QQHHTQNSQP QQQQQQQPQQ QMSQQQMSQH PRPQQGIPHP QQSQPQQQQQ QQQQLQQQQQ 

       250        260        270        280 
QQQQQPLTGI HQPHQQAFAN AASPYLNAEQ NAAYQQYFQE PQQGQY 

References and documentation are available.

Number of amino acids: 286

Molecular weight: 32801.56

Theoretical pI: 4.97

Amino acid composition: 
Ala (A)  15	  5.2%
Arg (R)   8	  2.8%
Asn (N)  16	  5.6%
Asp (D)  16	  5.6%
Cys (C)   1	  0.3%
Gln (Q)  77	 26.9%
Glu (E)  18	  6.3%
Gly (G)  15	  5.2%
His (H)  10	  3.5%
Ile (I)  10	  3.5%
Leu (L)  17	  5.9%
Lys (K)  11	  3.8%
Met (M)   6	  2.1%
Phe (F)   7	  2.4%
Pro (P)  19	  6.6%
Ser (S)  13	  4.5%
Thr (T)  12	  4.2%
Trp (W)   0	  0.0%
Tyr (Y)   5	  1.7%
Val (V)  10	  3.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 34
Total number of positively charged residues (Arg + Lys): 19

Atomic composition:

Carbon      C	      1387
Hydrogen    H	      2174
Nitrogen    N	       434
Oxygen      O	       478
Sulfur      S	         7

Formula: C1387H2174N434O478S7
Total number of atoms: 4480

Extinction coefficients:

This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient     7450
Abs 0.1% (=1 g/l)   0.227, assuming all pairs of Cys residues form cystines


Ext. coefficient     7450
Abs 0.1% (=1 g/l)   0.227, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 83.97
This classifies the protein as unstable.



Aliphatic index: 52.20

Grand average of hydropathicity (GRAVY): -1.416