ProtParam
User-provided sequence:
10 20 30 40 50 60
MSDLDEESQI ETQIDAPIED IIRGSELTTT TADKETLKSA NELLDSLEHS HRVDLSLHLY
70 80 90 100 110 120
SAYLLKRLLY KANEKKHFYE VNQFVKTQIK DNWTSWPNPN TIIDPSVDKL YEDIPEGIAN
130 140 150 160 170 180
VSVQPGEISN RALMHASDMM RVELDAQWQK FLSKSALDHD VTLDVDELNI PNEISRNILV
190 200 210 220 230 240
KLDSLFEGLH DKIAKENEFD VRQDKHSNNI RANQIDDEPM QANRRIKYTY HDLVSRGCEM
250 260 270 280 290 300
NEDMTDIYMK SLELYNDIPE KYKKRKFRLP KQILKKYHQP KKTSSYLKEL LSKTREDFIP
310 320 330 340 350 360
VEKLLKDKRL TSKDKSKLQR LNREETEDAL NKRTFFQVKG YLEDENEISD YELDDCLIEL
PNGNI
References and
documentation are available.
Number of amino acids: 365
Molecular weight: 42813.28
Theoretical pI: 5.21
Total number of negatively charged residues (Asp + Glu): 70
Total number of positively charged residues (Arg + Lys): 54
Atomic composition:
Carbon C 1886
Hydrogen H 3003
Nitrogen N 515
Oxygen O 600
Sulfur S 10
Formula: C
1886H
3003N
515O
600S
10
Total number of atoms: 6014
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 37485
Abs 0.1% (=1 g/l) 0.876, assuming all pairs of Cys residues form cystines
Ext. coefficient 37360
Abs 0.1% (=1 g/l) 0.873, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 38.36
This classifies the protein as stable.
Aliphatic index: 86.55
Grand average of hydropathicity (GRAVY): -0.832