ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSDLPIEFTE LVDLMSLGIS PQFLDFRSTT FESDHFVTVR ETKDGTNSVA IVDLAKGNEV 

        70         80         90        100        110        120 
TRKNMGGDSA IMHPSQMVIS VRANGTIVQI FNLETKSKLK SFTLDEPVIF WRWLSETTLG 

       130        140        150        160        170        180 
FVTARSILTS NVFDGNVNAK PQLLTLRHAN LNNTQIINFV ANKNLDWFAV VGILQENGRI 

       190        200        210        220        230        240 
AGRIQLFSKQ RNISQAIDGH VAIFTNILLE GNGSTPVQVF VTGNRNATTG AGELRIIEID 

       250        260        270        280        290        300 
HDASLPSQYQ KETTDIFFPP DATNDFPIAV QVSEKYGIIY LLTKYGFIHL YELETGTNLF 

       310        320        330        340        350        360 
VNRITAESVF TAAPYNHENG IACINKKGQV LAVEISTSQI VPYILNKLSN VALALIVATR 

       370        380        390        400        410        420 
GGLPGADDLF QKQFESLLLQ NDYQNAAKVA ASSTSLRNQN TINRLKNIQA PPGAISPILL 

       430        440        450        460        470        480 
YFSTLLDKGK LNKEETIELA RPVLQQDRKQ LFEKWLKEDK LECSEELGDI VKPFDTTLAL 

       490        500        510        520        530        540 
ACYLRAGAHA KVISCLAELQ QFEKIIPYCQ KVGYQPNFLV LISSLIRSSP DRASEFAVSL 

       550        560        570        580        590        600 
LQNPETASQI DIEKIADLFF SQNHIQQGTS LLLDALKGDT PDQGHLQTRV LEVNLLHAPQ 

       610        620        630        640        650        660 
VADAILGNNI FSHYDKPTIA SLSEKAGLYQ RALENYTDIK DIKRCVVHTN ALPIDWLVGY 

       670        680        690        700        710        720 
FGKLNVEQSL ACLKALMDNN IQANIQTVVQ VATKFSDLIG PSTLIKLFED YNATEGLYYY 

       730        740        750        760        770        780 
LASLVNLTED KDVVYKYIEA AAKMKQYREI ERIVKDNNVY DPERVKNFLK DANLEDQLPL 

       790        800        810        820        830        840 
VIVCDRFDFV HEMILYLYKS QNLKFIETYV QQVNPSKTAQ VVGALLDMDC DEAFIQSLLQ 

       850        860        870        880        890        900 
SVLGQVPINE LTTEVEKRNR LKILLPFLEQ SLSQGIQDQA VYNALAKIYI DSNNSPEKFL 

       910        920        930        940        950        960 
KENDQYDTLD VGHYCEKRDP YLAYIAYEKG QNDDDLIRIT NENSMYKYQA RYLLERSDLD 

       970        980        990       1000       1010       1020 
LWNKVLNQEN IHRRQLIDSV ISVGIPELTD PEPVSLTVQA FMTNGLKLEL IELLEKIILE 

      1030       1040       1050       1060       1070       1080 
PSPFNENVAL QGLLLLSAIK YEPTKVSSYI EKLDNYDADE IAPLCIEHDL KEEAFEIYDK 

      1090       1100       1110       1120       1130       1140 
HEMYGKALKV LIEDIMSLDR AASYADKINT PELWSQIGTA QLDGLRIPDA IESYIKAEDP 

      1150       1160       1170       1180       1190       1200 
SNYENVIDIA EQAGKYEELI PFLLMARKTL KEPKIDGALI LAYAELNKIH EIENLLAGSN 

      1210       1220       1230       1240       1250       1260 
VANLDHVGDK LFENKEYKAA RLCYSAVSNY SKLASTLVYL GDYQAAVDTA RKASNIKVWK 

      1270       1280       1290       1300       1310       1320 
LVNDACIEKK EFKLAQICGL NLIVHAEELD ELVERYESNG YFEELISLFE AGLGLERAHM 

      1330       1340       1350       1360       1370       1380 
GMFTELAILY SKYEPDKTFE HLKLFWSRIN IPKVIRAVEQ AHLWSELVFL YAHYDEWDNA 

      1390       1400       1410       1420       1430       1440 
ALTLIEKSTK DLDHAYFKEV VVKVSNLEIY YKAINFYVKF HPSLLVDLLT SLTPRLDIPR 

      1450       1460       1470       1480       1490       1500 
TVKIFSKSDN LPLIKPFLIN VLPKNNSVVN QAYHDLMIEE EDYKALQDAV DSYDKFDQLG 

      1510       1520       1530       1540       1550       1560 
LASRLESHKL IFFKKIGALL YRRNKKWAKS LSILKEEKLW KDAIETAAIS QDPKVVEALL 

      1570       1580       1590       1600       1610       1620 
TYFVETGNRE GFVALLYAAY NLVRIEFVLE ISWMNSLEDY IKPFEISIKK EQNDSIKKIT 

      1630       1640       1650 
EELAKKSGSN EEHKDGQPLM LMNSAMNVQP TGF 

References and documentation are available.

Number of amino acids: 1653

Molecular weight: 187234.23

Theoretical pI: 5.11

Amino acid composition: 
Ala (A) 127	  7.7%
Arg (R)  52	  3.1%
Asn (N) 101	  6.1%
Asp (D) 100	  6.0%
Cys (C)  14	  0.8%
Gln (Q)  74	  4.5%
Glu (E) 127	  7.7%
Gly (G)  67	  4.1%
His (H)  30	  1.8%
Ile (I) 128	  7.7%
Leu (L) 205	 12.4%
Lys (K) 118	  7.1%
Met (M)  21	  1.3%
Phe (F)  67	  4.1%
Pro (P)  58	  3.5%
Ser (S) 101	  6.1%
Thr (T)  76	  4.6%
Trp (W)  14	  0.8%
Tyr (Y)  70	  4.2%
Val (V) 103	  6.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 227
Total number of positively charged residues (Arg + Lys): 170

Atomic composition:

Carbon      C	      8468
Hydrogen    H	     13340
Nitrogen    N	      2176
Oxygen      O	      2530
Sulfur      S	        35

Formula: C8468H13340N2176O2530S35
Total number of atoms: 26549

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   182175
Abs 0.1% (=1 g/l)   0.973, assuming all pairs of Cys residues form cystines


Ext. coefficient   181300
Abs 0.1% (=1 g/l)   0.968, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 40.42
This classifies the protein as unstable.



Aliphatic index: 104.32

Grand average of hydropathicity (GRAVY): -0.167