ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSDNGSPAVL PKTEFNKYKI GKVKSTPAIQ RDAKTNLTYI KLRKRSSEKV YGCTVFQNHY 

        70         80         90        100        110        120 
REDEKLGQGT FGEVYKGIHL ETQRQVAMKK IIVSVEKDLF PITAQREITI LKRLNHKNII 

       130        140        150        160        170        180 
KLIEMVYDHS PDITNAASSN LHKSFYMILP YMVADLSGVL HNPRINLEMC DIKNMMLQIL 

       190        200        210        220        230        240 
EGLNYIHCAK FMHRDIKTAN ILIDHNGVLK LADFGLARLY YGCPPNLKYP GGAGSGAKYT 

       250        260        270        280        290        300 
SVVVTRWYRA PELVLGDKQY TTAVDIWGVG CVFAEFFEKK PILQGKTDID QGHVIFKLLG 

       310        320        330        340        350        360 
TPTEEDWAVA RYLPGAELTT TNYKPTLRER FGKYLSETGL DFLGQLLALD PYKRLTAMSA 

       370        380        390        400        410        420 
KHHPWFKEDP LPSEKITLPT EESHEADIKR YKEEMHQSLS QRVPTAPRGH IVEKGESPVV 

       430        440        450        460        470        480 
KNLGAIPRGP KKDDASFLPP SKNVLAKPPP SKIRELHQNP RPYHVNSGYA KTAIPPPAAP 

       490        500        510        520        530        540 
AGVNRYGPNN SSRNNRFSGN STAPNNSRNP VNRFHPETNV SSKYNKVPLP LGPQSRYQGN 

       550        560        570        580        590        600 
SNESRYKNSP NDSRYHNPRY VNKPETNFNR QPQKYSRQES NAPINKNYNP SNGSRNMAGD 

       610        620        630        640        650 
HHQGSRPSHP QFPISPSQGQ HQLTSKPIEK KNGSFKDERA KPDESKEFQN SDIADLY 

References and documentation are available.

Number of amino acids: 657

Molecular weight: 74239.25

Theoretical pI: 9.55

Amino acid composition: 
Ala (A)  38	  5.8%
Arg (R)  36	  5.5%
Asn (N)  48	  7.3%
Asp (D)  28	  4.3%
Cys (C)   5	  0.8%
Gln (Q)  25	  3.8%
Glu (E)  37	  5.6%
Gly (G)  41	  6.2%
His (H)  23	  3.5%
Ile (I)  35	  5.3%
Leu (L)  49	  7.5%
Lys (K)  57	  8.7%
Met (M)  12	  1.8%
Phe (F)  21	  3.2%
Pro (P)  55	  8.4%
Ser (S)  48	  7.3%
Thr (T)  32	  4.9%
Trp (W)   4	  0.6%
Tyr (Y)  31	  4.7%
Val (V)  32	  4.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 65
Total number of positively charged residues (Arg + Lys): 93

Atomic composition:

Carbon      C	      3304
Hydrogen    H	      5181
Nitrogen    N	       945
Oxygen      O	       972
Sulfur      S	        17

Formula: C3304H5181N945O972S17
Total number of atoms: 10419

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    68440
Abs 0.1% (=1 g/l)   0.922, assuming all pairs of Cys residues form cystines


Ext. coefficient    68190
Abs 0.1% (=1 g/l)   0.919, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 46.69
This classifies the protein as unstable.



Aliphatic index: 69.77

Grand average of hydropathicity (GRAVY): -0.775