ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSDNPEMKPH GTSKEIVESV TDATSKAIDK LQEELHKDAS ESVTPVTKES TAATKESRKY 

        70         80         90        100        110        120 
NFFIRTVWTF VMISGFFITL ASGHAWCIVL ILGCQIATFK ECIAVTSASG REKNLPLTKT 

       130        140        150        160        170        180 
LNWYLLFTTI YYLDGKSLFK FFQATFYEYP VLNFIVTNHK FICYCLYLMG FVLFVCSLRK 

       190        200        210        220        230        240 
GFLKFQFGSL CVTHMVLLLV VFQAHLIIKN VLNGLFWFLL PCGLVIVNDI FAYLCGITFG 

       250        260        270        280        290        300 
KTKLIEISPK KTLEGFLGAW FFTALASIIL TRILSPYTYL TCPVEDLHTN FFSNLTCELN 

       310        320        330        340        350        360 
PVFLPQVYRL PPIFFDKVQI NSITVKPIYF HALNLATFAS LFAPFGGFFA SGLKRTFKVK 

       370        380        390        400        410        420 
DFGHSIPGHG GITDRVDCQF IMGSFANLYY ETFISEHRIT VDTVLSTILM NLNDKQIIEL 

       430        440        450 
IDILIRFLSK KGIISAKNFE KLADIFNVTK KSLTNHS 

References and documentation are available.

Number of amino acids: 457

Molecular weight: 51822.94

Theoretical pI: 8.84

Amino acid composition: 
Ala (A)  24	  5.3%
Arg (R)  10	  2.2%
Asn (N)  20	  4.4%
Asp (D)  15	  3.3%
Cys (C)  12	  2.6%
Gln (Q)   9	  2.0%
Glu (E)  19	  4.2%
Gly (G)  24	  5.3%
His (H)  12	  2.6%
Ile (I)  40	  8.8%
Leu (L)  54	 11.8%
Lys (K)  33	  7.2%
Met (M)   7	  1.5%
Phe (F)  45	  9.8%
Pro (P)  16	  3.5%
Ser (S)  30	  6.6%
Thr (T)  38	  8.3%
Trp (W)   5	  1.1%
Tyr (Y)  15	  3.3%
Val (V)  29	  6.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 34
Total number of positively charged residues (Arg + Lys): 43

Atomic composition:

Carbon      C	      2427
Hydrogen    H	      3730
Nitrogen    N	       578
Oxygen      O	       638
Sulfur      S	        19

Formula: C2427H3730N578O638S19
Total number of atoms: 7392

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    50600
Abs 0.1% (=1 g/l)   0.976, assuming all pairs of Cys residues form cystines


Ext. coefficient    49850
Abs 0.1% (=1 g/l)   0.962, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 21.85
This classifies the protein as stable.



Aliphatic index: 103.87

Grand average of hydropathicity (GRAVY): 0.388