ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSDQDNTQTS SSKLAPHHNI FMANLESSPT KDRNTSSQNA SSSRVIESLH DQIDMLTKTN 

        70         80         90        100        110        120 
LQLTTQSQNL LSKLELAQSK ESKLLENLNL LKNENENLNS IFERKNKKLK ELEKDYSELS 

       130        140        150        160        170        180 
NRYNEQKEKM DQLSKLAKNS SAIEQSCSEK LQNMEVNYNS LLESQNLYRD HYSDEISKLN 

       190        200        210        220        230        240 
EKIGLLELEL SNQNLNYGSD TSSNSDIELN LNKFNDSVKD LKSLETEKDS KLSKIITHSL 

       250        260        270        280        290        300 
DELNLQSWLN LYQTNENLIS TFAEKMDLKD VLKRNDEKIS NKGAVVQTLK KNVQTQVESN 

       310        320        330        340        350        360 
NADALSSNNA QDMLPIKMVK LRKTPNTNDS SSNGNSSNNK RRSFYTASPL LSSGSIPKSA 

       370        380        390        400        410        420 
SPVLPGVKRT ASVRKPSSSS SKTNVTHNND PSTSPTISVP PGVTRTVSST HKKKGNSMVV 


HGAQS 

References and documentation are available.

Number of amino acids: 425

Molecular weight: 47416.69

Theoretical pI: 8.74

Amino acid composition: 
Ala (A)  15	  3.5%
Arg (R)  12	  2.8%
Asn (N)  50	 11.8%
Asp (D)  22	  5.2%
Cys (C)   1	  0.2%
Gln (Q)  21	  4.9%
Glu (E)  29	  6.8%
Gly (G)   9	  2.1%
His (H)   8	  1.9%
Ile (I)  15	  3.5%
Leu (L)  51	 12.0%
Lys (K)  43	 10.1%
Met (M)   9	  2.1%
Phe (F)   5	  1.2%
Pro (P)  13	  3.1%
Ser (S)  69	 16.2%
Thr (T)  26	  6.1%
Trp (W)   1	  0.2%
Tyr (Y)   8	  1.9%
Val (V)  18	  4.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 51
Total number of positively charged residues (Arg + Lys): 55

Atomic composition:

Carbon      C	      2017
Hydrogen    H	      3320
Nitrogen    N	       592
Oxygen      O	       702
Sulfur      S	        10

Formula: C2017H3320N592O702S10
Total number of atoms: 6641

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    17420
Abs 0.1% (=1 g/l)   0.367, assuming all pairs of Cys residues form cystines


Ext. coefficient    17420
Abs 0.1% (=1 g/l)   0.367, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 51.91
This classifies the protein as unstable.



Aliphatic index: 76.38

Grand average of hydropathicity (GRAVY): -0.908