ProtParam
User-provided sequence:
10 20 30 40 50 60
MSDQPPAENA DVRQRRAPES PVTERLRAPA RDDARPTSDE SDMEGILQDE DSDAGSKNKE
70 80 90 100 110 120
EKLERLTQAI PQDKGSLGVF ADSMLEALPP RWRNWVVRGL FSIIMISTFT FIVTRGATWL
130 140 150 160 170 180
MFLVFLIQFK CFQEIISIGL AVYRLYDFPW FRALSWYFLL TSNYFFFGES LIDYWGIVLK
190 200 210 220 230 240
KDNFLHFLVA YHRLVSFALY CIGFVSFVLS LRKGYYMRQF SLFAWTHLTL LLIVSQSFFI
250 260 270 280 290 300
IQNIFQGLIW FLAPVAMIIC CDIMSYMFGF FWGKTPLIKL SPKKTWEGFI GGAFSTVVFG
310 320 330 340 350 360
ILLSLALYNR PFFVCPVQHY QTDSSNCTIP LAFQLQDYPV PRPFSFVYKI LRKEPIIQLC
370 380 390 400 410 420
PFVFHSIALS LFASILGPFG GFFASGFKRA FKIKDFGDVI PGHGGLMDRF DCQLLMGTFV
430 440 450 460
MVYIHSFIRV PDASKLLKQI MTLEPQDQLN IFNLLQSELS KTGLI
References and
documentation are available.
Number of amino acids: 465
Molecular weight: 53459.67
Theoretical pI: 8.51
Total number of negatively charged residues (Asp + Glu): 39
Total number of positively charged residues (Arg + Lys): 43
Atomic composition:
Carbon C 2510
Hydrogen H 3791
Nitrogen N 609
Oxygen O 643
Sulfur S 21
Formula: C
2510H
3791N
609O
643S
21
Total number of atoms: 7574
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 77850
Abs 0.1% (=1 g/l) 1.456, assuming all pairs of Cys residues form cystines
Ext. coefficient 77350
Abs 0.1% (=1 g/l) 1.447, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 43.16
This classifies the protein as unstable.
Aliphatic index: 98.97
Grand average of hydropathicity (GRAVY): 0.310