ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSDRDQIEPV TNALDAESDS SDDFGNFSDA SVENDLYNQN STLTTSSESV VDNCLNKILP 

        70         80         90        100        110        120 
KGEFDLEEET IKNDCFKLSK LIEDERPHVI YEQLVQLDPV LQPFIWNKSH IRRNLLHILR 

       130        140        150        160        170        180 
LSDNNGSEGV GTKREEEPLN DELFKRICDA VEKNEQTATG LFLRDNFKID YTPPMTLKSL 

       190        200        210        220        230        240 
QKEEEREQEQ HIPQLLMADF TSMDEESLRQ YHDTLCQSID FLVSKSRSLK KQQRDLLKDK 

       250        260        270        280 
TTFENVVTNL TGHTQRLQRD EIALYNKKRN KKKRFSWVGY 

References and documentation are available.

Number of amino acids: 280

Molecular weight: 32639.44

Theoretical pI: 4.99

Amino acid composition: 
Ala (A)   7	  2.5%
Arg (R)  16	  5.7%
Asn (N)  19	  6.8%
Asp (D)  25	  8.9%
Cys (C)   4	  1.4%
Gln (Q)  16	  5.7%
Glu (E)  27	  9.6%
Gly (G)   8	  2.9%
His (H)   6	  2.1%
Ile (I)  13	  4.6%
Leu (L)  32	 11.4%
Lys (K)  22	  7.9%
Met (M)   4	  1.4%
Phe (F)  12	  4.3%
Pro (P)   9	  3.2%
Ser (S)  22	  7.9%
Thr (T)  17	  6.1%
Trp (W)   2	  0.7%
Tyr (Y)   6	  2.1%
Val (V)  13	  4.6%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 52
Total number of positively charged residues (Arg + Lys): 38

Atomic composition:

Carbon      C	      1422
Hydrogen    H	      2257
Nitrogen    N	       399
Oxygen      O	       465
Sulfur      S	         8

Formula: C1422H2257N399O465S8
Total number of atoms: 4551

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    20190
Abs 0.1% (=1 g/l)   0.619, assuming all pairs of Cys residues form cystines


Ext. coefficient    19940
Abs 0.1% (=1 g/l)   0.611, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 56.67
This classifies the protein as unstable.



Aliphatic index: 78.64

Grand average of hydropathicity (GRAVY): -0.856