ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSDREFVTVD PVTIIIKECI NLSTAMRKYS KFTSQSGVAA LLGGGSEIFS NQDDYLAHTF 

        70         80         90        100        110        120 
NNLNTNKHND PFLSGFIQLR LMLNKLKNLD NIDSLTILQP FLLIVSTSSI SGYITSLALD 

       130        140        150        160        170        180 
SLQKFFTLNI INESSQNYIG AHRATVNALT HCRFEGSQQL SDDSVLLKVV FLLRSIVDSP 

       190        200        210        220        230        240 
YGDLLSNSII YDVLQTILSL ACNNRRSEVL RNAAQSTMIA VTVKIFSKLK TIEPVNVNQI 

       250        260        270        280        290        300 
YINDESYTND VLKADTIGTN VESKEEGSQE DPIGMKVNNE EAISEDDGIE EEHIHSEKST 

       310        320        330        340        350        360 
NGAEQLDIVQ KTTRSNSRIQ AYADDNYGLP VVRQYLNLLL SLIAPENELK HSYSTRIFGL 

       370        380        390        400        410        420 
ELIQTALEIS GDRLQLYPRL FTLISDPIFK SILFIIQNTT KLSLLQATLQ LFTTLVVILG 

       430        440        450        460        470        480 
NNLQLQIELT LTRIFSILLD DGTANNSSSE NKNKPSIIKE LLIEQISILW TRSPSFFTST 

       490        500        510        520        530        540 
FINFDCNLDR ADVSINFLKA LTKLALPESA LTTTESVPPI CLEGLVSLVD DMFDHMKDID 

       550        560        570        580        590        600 
REEFGRQKNE MEILKKRDRK TEFIECTNAF NEKPKKGIPM LIEKGFIASD SDKDIAEFLF 

       610        620        630        640        650        660 
NNNNRMNKKT IGLLLCHPDK VSLLNEYIRL FDFSGLRVDE AIRILLTKFR LPGESQQIER 

       670        680        690        700        710        720 
IIEAFSSAYC ENQDYDPSKI SDNAEDDIST VQPDADSVFI LSYSIIMLNT DLHNPQVKEH 

       730        740        750        760        770        780 
MSFEDYSGNL KGCCNHKDFP FWYLDRIYCS IRDKEIVMPE EHHGNEKWFE DAWNNLISST 

       790        800        810        820        830        840 
TVITEIKKDT QSVMDKLTPL ELLNFDRAIF KQVGPSIVST LFNIYVVASD DHISTRMITS 

       850        860        870        880        890        900 
LDKCSYISAF FDFKDLFNDI LNSIAKGTTL INSSHDDELS TLAFEYGPMP LVQIKFEDTN 

       910        920        930        940        950        960 
TEIPVSTDAV RFGRSFKGQL NTVVFFRIIR RNKDPKIFSK ELWLNIVNII LTLYEDLILS 

       970        980        990       1000       1010       1020 
PDIFPDLQKR LKLSNLPKPS PEISINKSKE SKGLLSTFAS YLKGDEEPTE EEIKSSKKAM 

      1030       1040       1050       1060       1070       1080 
ECIKSSNIAA SVFGNESNIT ADLIKTLLDS AKTEKNADNS RYFEAELLFI IELTIALFLF 

      1090       1100       1110       1120       1130       1140 
CKEEKELGKF ILQKVFQLSH TKGLTKRTVR RMLTYKILLI SLCADQTEYL SKLINDELLK 

      1150       1160       1170       1180       1190       1200 
KGDIFTQKFF ATNQGKEFLK RLFSLTESEF YRGFLLGNEN FWKFLRKVTA MKEQSESIFE 

      1210       1220       1230       1240       1250       1260 
YLNESIKTDS NILTNENFMW VLGLLDEISS MGAVGNHWEI EYKKLTESGH KIDKENPYKK 

      1270       1280       1290       1300       1310       1320 
SIELSLKSIQ LTSHLLEDNN DLRKNEIFAI IQALAHQCIN PCKQISEFAV VTLEQTLINK 

      1330       1340       1350       1360       1370       1380 
IEIPTNEMES VEELIEGGLL PLLNSSETQE DQKILISSIL TIISNVYLHY LKLGKTSNET 

      1390       1400       1410       1420       1430       1440 
FLKILSIFNK FVEDSDIEKK LQQLILDKKS IEKGNGSSSH GSAHEQTPES NDVEIEATAP 

      1450 
IDDNTDDDNK PKLSDVEKD 

References and documentation are available.

Number of amino acids: 1459

Molecular weight: 165667.88

Theoretical pI: 5.03

Amino acid composition: 
Ala (A)  60	  4.1%
Arg (R)  46	  3.2%
Asn (N)  99	  6.8%
Asp (D)  95	  6.5%
Cys (C)  17	  1.2%
Gln (Q)  51	  3.5%
Glu (E) 116	  8.0%
Gly (G)  53	  3.6%
His (H)  24	  1.6%
Ile (I) 139	  9.5%
Leu (L) 176	 12.1%
Lys (K) 110	  7.5%
Met (M)  22	  1.5%
Phe (F)  78	  5.3%
Pro (P)  43	  2.9%
Ser (S) 138	  9.5%
Thr (T)  91	  6.2%
Trp (W)   8	  0.5%
Tyr (Y)  34	  2.3%
Val (V)  59	  4.0%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 211
Total number of positively charged residues (Arg + Lys): 156

Atomic composition:

Carbon      C	      7412
Hydrogen    H	     11785
Nitrogen    N	      1913
Oxygen      O	      2295
Sulfur      S	        39

Formula: C7412H11785N1913O2295S39
Total number of atoms: 23444

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    95660
Abs 0.1% (=1 g/l)   0.577, assuming all pairs of Cys residues form cystines


Ext. coefficient    94660
Abs 0.1% (=1 g/l)   0.571, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 45.34
This classifies the protein as unstable.



Aliphatic index: 100.04

Grand average of hydropathicity (GRAVY): -0.232