ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSDSFANVPG GSQKLNSGYN IPFVGFGTYK VTGENVPPAI DAALTAGYRM FDTAKYYLNE 

        70         80         90        100        110        120 
KELGEALKIL LPKHGLSRSD VFLTSKFFPE SKNCREACRG FVEESLQSLQ TDYIDMYLVH 

       130        140        150        160        170        180 
YPKPNDSDND DVNNAEYRKI AYEVLEEAKA AGKVRSIGVS NYEIVHLEEL KTYAKVPPCA 

       190        200        210        220        230        240 
NQLEYHPHFA RIPLQKYCKE KNIFFQAFSS LARHEPKLIE DPVVVELAKK HNTSVPLVLL 

       250        260        270        280        290 
AWALRQNVGI VPKSVTPSRI VENFKVIDIA LTPEDIQSLT ALDRGQHYIR CTGWLVK 

References and documentation are available.

Number of amino acids: 297

Molecular weight: 33475.29

Theoretical pI: 6.72

Amino acid composition: 
Ala (A)  23	  7.7%
Arg (R)  12	  4.0%
Asn (N)  16	  5.4%
Asp (D)  14	  4.7%
Cys (C)   5	  1.7%
Gln (Q)   9	  3.0%
Glu (E)  22	  7.4%
Gly (G)  15	  5.1%
His (H)   8	  2.7%
Ile (I)  16	  5.4%
Leu (L)  28	  9.4%
Lys (K)  23	  7.7%
Met (M)   3	  1.0%
Phe (F)  13	  4.4%
Pro (P)  18	  6.1%
Ser (S)  19	  6.4%
Thr (T)  12	  4.0%
Trp (W)   2	  0.7%
Tyr (Y)  15	  5.1%
Val (V)  24	  8.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 36
Total number of positively charged residues (Arg + Lys): 35

Atomic composition:

Carbon      C	      1515
Hydrogen    H	      2359
Nitrogen    N	       399
Oxygen      O	       441
Sulfur      S	         8

Formula: C1515H2359N399O441S8
Total number of atoms: 4722

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    33600
Abs 0.1% (=1 g/l)   1.004, assuming all pairs of Cys residues form cystines


Ext. coefficient    33350
Abs 0.1% (=1 g/l)   0.996, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 45.11
This classifies the protein as unstable.



Aliphatic index: 88.96

Grand average of hydropathicity (GRAVY): -0.294