ProtParam
User-provided sequence:
10 20 30 40 50 60
MSDSGVFAFE LGDDLATRCG NTLTGVFVPG SRIALSAYRH VTHSSRDEPT ENAGQVPVLI
70 80 90 100 110 120
YMAQESTLFD EPVLRSVLAE ANATFATLQQ LGSRATRAEY VNISRAYRSI VRSCLEKLEQ
130 140 150 160 170 180
AKKSPEVQTD EARLQRLCDA IVVFYAAECL WHLFEILYIQ SNQLVVPQLL DWARFHSPHA
190 200 210 220 230 240
EDRATDLLLM VEEASESDDY WSIVKSLIML GEIDVTRAVL SQNRKAGQTS FKAAEQILKS
250 260 270 280 290 300
MPVYQEGYAL QKFHSQWEFW HVDTERKIQS GLFATEPELE QLIRLVAGDS EQWDAGIKES
310 320 330 340 350 360
QDFYEYLPGY LLFTKPTCKP FELKIAAAKW LNRWCLLRPE REQCSMNRMV SQLMDHDLRL
370 380 390 400 410 420
FIYDAQKLND THWFSTHLID LIHHCGQLKS YFDQNNIDLP ALRHSMIYEY GSYLMTSHNM
430 440 450 460 470 480
WQLGIDYLDC CKQEGQAAIE LLLPRITLRS ERQATKLINL ARQRGLISVE REICKVLSKR
490 500 510 520 530 540
SYDNERYGNA LEWAIRSKDV LLVTAVADFI LKHYSKTGCM LCPDTIANVG GRMFASPRLV
550 560 570 580 590 600
FLSKYFEFYE FYRTRDFLSA SELLVNLLES KITPDYFWPS LLIDSMPLLE SKDPKIFAKE
610 620 630 640 650 660
TVAILHHIET DLVPIIERDV SKYGKHHTET VFKDYRVENV DEIMNLLRLA CARNLARALI
IENTLPVV
References and
documentation are available.
Number of amino acids: 668
Molecular weight: 76914.18
Theoretical pI: 5.70
Total number of negatively charged residues (Asp + Glu): 89
Total number of positively charged residues (Arg + Lys): 73
Atomic composition:
Carbon C 3455
Hydrogen H 5394
Nitrogen N 926
Oxygen O 1005
Sulfur S 29
Formula: C
3455H
5394N
926O
1005S
29
Total number of atoms: 10809
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 107105
Abs 0.1% (=1 g/l) 1.393, assuming all pairs of Cys residues form cystines
Ext. coefficient 106230
Abs 0.1% (=1 g/l) 1.381, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 50.50
This classifies the protein as unstable.
Aliphatic index: 95.94
Grand average of hydropathicity (GRAVY): -0.185