ProtParam
User-provided sequence:
10 20 30 40 50 60
MSDSPIELMR STLAGFQIAP DVKTISNIQD IRAQIQSFRE KELEGSQTKF KVLSRKLEIS
70 80 90 100 110 120
TQAMESLKQT AESSQHAEEI LSREKEKFRI AKLLNITENE IMSLESQLQK MKEQLLQLEE
130 140 150 160 170 180
RENTSEDICQ SEENANMLKL NFYHSLGFDL ETAENTGNKR VIIHTENDLQ TVQISNKYSP
190
YFYSNYFWDL LDDSKDKK
References and
documentation are available.
Number of amino acids: 198
Molecular weight: 23038.90
Theoretical pI: 5.00
Total number of negatively charged residues (Asp + Glu): 34
Total number of positively charged residues (Arg + Lys): 25
Atomic composition:
Carbon C 1005
Hydrogen H 1611
Nitrogen N 273
Oxygen O 331
Sulfur S 7
Formula: C
1005H
1611N
273O
331S
7
Total number of atoms: 3227
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 12950
Abs 0.1% (=1 g/l) 0.562, assuming all pairs of Cys residues form cystines
Ext. coefficient 12950
Abs 0.1% (=1 g/l) 0.562, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 68.15
This classifies the protein as unstable.
Aliphatic index: 81.31
Grand average of hydropathicity (GRAVY): -0.743