ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSDSSSSDGE DNQKFLGNRK KNLTKKKVVT EEIEKDDEED VKEKSFAELG VSQPLCDACQ 

        70         80         90        100        110        120 
RLGWMKPSKI QQAALPHALQ GKDVIGLAET GSGKTGAFAI PVLQSLLDHP QAFFCLVLTP 

       130        140        150        160        170        180 
TRELAFQIGQ QFEALGSGIG LIAAVIVGGV DMAAQAMALA RRPHIIVATP GRLVDHLENT 

       190        200        210        220        230        240 
KGFNLKALKF LIMDEADRIL NMDFEVELDK ILKVIPRERR TYLFSATMTK KVSKLERASL 

       250        260        270        280        290        300 
RDPARVSVSS RYKTVDNLKQ HYIFVPNKYK ETYLVYLLNE HAGNSAIVFC ATCATTMQIA 

       310        320        330        340        350        360 
VMLRQLGMQA VPLHGQMSQE KRLGSLNKFK SKAREILVCT DVAARGLDIP HVDMVINYDM 

       370        380        390        400        410        420 
PSQSKDYVHR VGRTARAGRS GIAITVVTQY DVEAYQKIEA NLGKKLDEYK CVENEVMVLV 

       430        440        450        460        470        480 
ERTQEATENA RIEMKEMDEK KKSGKKRRQN DDFGDTEESG GRFKMGIKSM GGRGGSGGGR 


GGKKKKMSK 

References and documentation are available.

Number of amino acids: 489

Molecular weight: 54227.59

Theoretical pI: 9.24

Amino acid composition: 
Ala (A)  40	  8.2%
Arg (R)  29	  5.9%
Asn (N)  16	  3.3%
Asp (D)  28	  5.7%
Cys (C)   7	  1.4%
Gln (Q)  23	  4.7%
Glu (E)  34	  7.0%
Gly (G)  40	  8.2%
His (H)   9	  1.8%
Ile (I)  25	  5.1%
Leu (L)  42	  8.6%
Lys (K)  47	  9.6%
Met (M)  19	  3.9%
Phe (F)  16	  3.3%
Pro (P)  14	  2.9%
Ser (S)  29	  5.9%
Thr (T)  23	  4.7%
Trp (W)   1	  0.2%
Tyr (Y)  11	  2.2%
Val (V)  36	  7.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 62
Total number of positively charged residues (Arg + Lys): 76

Atomic composition:

Carbon      C	      2372
Hydrogen    H	      3879
Nitrogen    N	       681
Oxygen      O	       716
Sulfur      S	        26

Formula: C2372H3879N681O716S26
Total number of atoms: 7674

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    22265
Abs 0.1% (=1 g/l)   0.411, assuming all pairs of Cys residues form cystines


Ext. coefficient    21890
Abs 0.1% (=1 g/l)   0.404, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 40.59
This classifies the protein as unstable.



Aliphatic index: 82.97

Grand average of hydropathicity (GRAVY): -0.399