ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSDTEAPVEV QEDFEVVEEF TPVVLATPIP EEVQQAQTEI KLFNKWSFEE VEVKDASLVD 

        70         80         90        100        110        120 
YVQVRQPIFV AHTAGRYANK RFRKAQCPII ERLTNSLMMN GRNNGKKLKA VRIIKHTLDI 

       130        140        150        160        170        180 
INVLTDQNPI QVVVDAITNT GPREDTTRVG GGGAARRQAV DVSPLRRVNQ AIALLTIGAR 

       190        200        210        220 
EAAFRNIKTI AETLAEELIN AAKGSSTSYA IKKKDELERV AKSNR 

References and documentation are available.

Number of amino acids: 225

Molecular weight: 25038.61

Theoretical pI: 8.62

Amino acid composition: 
Ala (A)  24	 10.7%
Arg (R)  17	  7.6%
Asn (N)  13	  5.8%
Asp (D)  10	  4.4%
Cys (C)   1	  0.4%
Gln (Q)  11	  4.9%
Glu (E)  20	  8.9%
Gly (G)  10	  4.4%
His (H)   2	  0.9%
Ile (I)  17	  7.6%
Leu (L)  14	  6.2%
Lys (K)  15	  6.7%
Met (M)   3	  1.3%
Phe (F)   7	  3.1%
Pro (P)   9	  4.0%
Ser (S)   9	  4.0%
Thr (T)  16	  7.1%
Trp (W)   1	  0.4%
Tyr (Y)   3	  1.3%
Val (V)  23	 10.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 30
Total number of positively charged residues (Arg + Lys): 32

Atomic composition:

Carbon      C	      1099
Hydrogen    H	      1806
Nitrogen    N	       320
Oxygen      O	       338
Sulfur      S	         4

Formula: C1099H1806N320O338S4
Total number of atoms: 3567

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient     9970
Abs 0.1% (=1 g/l)   0.398, assuming all pairs of Cys residues form cystines


Ext. coefficient     9970
Abs 0.1% (=1 g/l)   0.398, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 36.32
This classifies the protein as stable.



Aliphatic index: 94.04

Grand average of hydropathicity (GRAVY): -0.332