ProtParam
User-provided sequence:
10 20 30 40 50 60
MSDTEEQEYE EEQAEDEEAV EEEAPEEPEP VAEREEERPK PSRPVVPPLI PPKIPEGERV
70 80 90 100 110 120
DFDDIHRKRM EKDLLELQTL IDVHFEQRKK EEEELIALKD RIERRRAERA EQQRFRTEKE
130 140 150 160 170 180
RERQAKLAEE KMRKEEEEAK KRAEDDAKKK KVLSNMGAHF GGYLVKAEQK RGKRQTGREM
190 200 210 220 230 240
KLRILSERKK PLNIDYMGED QLREKAQELS EWIHQLESEK FDLMEKLKQQ KYEINVLYNR
250 260
ISHAQKFRKG AGKGRVGGRW K
References and
documentation are available.
Number of amino acids: 261
Molecular weight: 31215.23
Theoretical pI: 5.85
Total number of negatively charged residues (Asp + Glu): 65
Total number of positively charged residues (Arg + Lys): 60
Atomic composition:
Carbon C 1351
Hydrogen H 2200
Nitrogen N 408
Oxygen O 427
Sulfur S 7
Formula: C
1351H
2200N
408O
427S
7
Total number of atoms: 4393
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 18450
Abs 0.1% (=1 g/l) 0.591
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 71.76
This classifies the protein as unstable.
Aliphatic index: 62.45
Grand average of hydropathicity (GRAVY): -1.445