ProtParam
User-provided sequence:
10 20 30 40 50 60
MSDTEEQEYE EEQAEDEEAV EEEEAPEEPE PVAEREEERP KPSRPVVPPL IPPKIPEGER
70 80 90 100 110 120
VDFDDIHRKR MEKDLLELQT LIDVHFEQRK KEEEELIALK DRIERRRAER AEQQRFRTEK
130 140 150 160 170 180
ERERQAKLAE EKMRKEEEEA KKRAEDDAKK KKVLSNMGAH FGGYLVKAEQ KRGKRQTGRE
190 200 210 220 230 240
MKLRILSERK KPLNIDYMGE DQLREKAQEL SEWIHQLESE KFDLMEKLKQ QKYEINVLYN
250 260
RISHAQKFRK GAGKGRVGGR WK
References and
documentation are available.
Number of amino acids: 262
Molecular weight: 31344.34
Theoretical pI: 5.74
Total number of negatively charged residues (Asp + Glu): 66
Total number of positively charged residues (Arg + Lys): 60
Atomic composition:
Carbon C 1356
Hydrogen H 2207
Nitrogen N 409
Oxygen O 430
Sulfur S 7
Formula: C
1356H
2207N
409O
430S
7
Total number of atoms: 4409
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 18450
Abs 0.1% (=1 g/l) 0.589
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 72.77
This classifies the protein as unstable.
Aliphatic index: 62.21
Grand average of hydropathicity (GRAVY): -1.453