ProtParam
User-provided sequence:
10 20 30 40 50 60
MSDTEKDKDV PMVDSHEATE EPPTTSTNTP SFPHLAQEQA KEESATLGAE VAHKKINYEQ
70 80 90 100 110 120
EAQKLEEKAL RFLAKQTHPV IIPSFASWFD ISKIHEIEKR SNPDFFNDSS RFKTPKAYKD
130 140 150 160 170 180
TRNFIINTYR LSPYEYLTIT AVRRNVAMDV ASIVKIHAFL EKWGLINYQI DPRTKPSLIG
190 200 210 220 230 240
PSFTGHFQVV LDTPQGLKPF LPENVIKQEV EGGDGAEPQV KKEFPVNLTI KKNVYDSAQD
250 260 270 280 290 300
FNALQDESRN SRQIHKVYIC HTCGNESINV RYHNLRARDT NLCSRCFQEG HFGANFQSSD
310 320 330 340 350 360
FIRLENNGNS VKKNWSDQEM LLLLEGIEMY EDQWEKIADH VGGHKRVEDC IEKFLSLPIE
370 380 390 400 410 420
DNYIREVVGS TLNGKGGDSR DGSVSGSKLM ECVNDAVQTL LQGDDKLGKV SDKSREISEK
430 440 450 460 470 480
YIEESQAIIQ ELVKLTMEKL ESKFTKLCDL ETQLEMEKLK YVKESEKMLN DRLSLSKQIL
490 500 510 520 530 540
DLNKSLEELN VSKKLVLISE QVDSGIQLVE KDQEGDDEDG NTATGHGVKR VGKEGEEVGE
550
GDSIAKLQPQ VYKPWSL
References and
documentation are available.
Number of amino acids: 557
Molecular weight: 63168.05
Theoretical pI: 5.24
Total number of negatively charged residues (Asp + Glu): 94
Total number of positively charged residues (Arg + Lys): 72
Atomic composition:
Carbon C 2778
Hydrogen H 4403
Nitrogen N 761
Oxygen O 887
Sulfur S 16
Formula: C
2778H
4403N
761O
887S
16
Total number of atoms: 8845
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 48735
Abs 0.1% (=1 g/l) 0.772, assuming all pairs of Cys residues form cystines
Ext. coefficient 48360
Abs 0.1% (=1 g/l) 0.766, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 41.82
This classifies the protein as unstable.
Aliphatic index: 79.95
Grand average of hydropathicity (GRAVY): -0.667