ProtParam
User-provided sequence:
10 20 30 40 50 60
MSDVFDGYER QYCELSASLS KKCSSAISLD GEQKKQKLSE IKSGLENAEV LIRKMDLEAR
70 80 90 100 110 120
TLPPNLKSSL LVKLREFKSD LNNFKTEVKR ITSGQLNAAA RDELLEAGMA DTKTASADQR
130 140 150 160 170 180
ARLMMSTERL GRTTDRVKDS RRTMMETEEI GVSILQDLHG QRQSLLRAHE TLHGVDDNIG
190 200 210 220
KSKKILTDMT RRMNKNKWTI GAIIIALIAA IFIILYFKLT K
References and
documentation are available.
Number of amino acids: 221
Molecular weight: 24950.87
Theoretical pI: 9.47
Total number of negatively charged residues (Asp + Glu): 30
Total number of positively charged residues (Arg + Lys): 38
Atomic composition:
Carbon C 1081
Hydrogen H 1814
Nitrogen N 316
Oxygen O 335
Sulfur S 11
Formula: C
1081H
1814N
316O
335S
11
Total number of atoms: 3557
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 10095
Abs 0.1% (=1 g/l) 0.405, assuming all pairs of Cys residues form cystines
Ext. coefficient 9970
Abs 0.1% (=1 g/l) 0.400, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 39.17
This classifies the protein as stable.
Aliphatic index: 92.76
Grand average of hydropathicity (GRAVY): -0.436