ProtParam
User-provided sequence:
10 20 30 40 50 60
MSDVFEGYER QYCELSTNLS RKCHSASVLS NGEEKKGKIA EIKSGIDEAD VLIRKMDLEA
70 80 90 100 110 120
RSLQPSAKAV CLSKLREYKS DLNQLKKEFK RVSSADAKPS SREELMESGM ADLHAVSADQ
130 140 150 160 170 180
RGRLAMSVER LDQSSDRIRE SRRLMLETEE VGISIVQDLS QQRQTLLHAH NKLHGVDDAI
190 200 210 220
DKSKKVLTAM SRRMTRNKWI ITSVIVALVL AIILIISYKL SH
References and
documentation are available.
Number of amino acids: 222
Molecular weight: 25056.83
Theoretical pI: 9.06
Total number of negatively charged residues (Asp + Glu): 32
Total number of positively charged residues (Arg + Lys): 37
Atomic composition:
Carbon C 1078
Hydrogen H 1808
Nitrogen N 322
Oxygen O 339
Sulfur S 11
Formula: C
1078H
1808N
322O
339S
11
Total number of atoms: 3558
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 11585
Abs 0.1% (=1 g/l) 0.462, assuming all pairs of Cys residues form cystines
Ext. coefficient 11460
Abs 0.1% (=1 g/l) 0.457, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 44.93
This classifies the protein as unstable.
Aliphatic index: 96.22
Grand average of hydropathicity (GRAVY): -0.424