ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSDVSADGGF LSAEQATTPV AIPTPYPSLT VSASYKEKSS GRRRPVRPSF DAAADNEFIT 

        70         80         90        100        110        120 
LLHGSDPVKV ELNRLENEVR DKDRELSEAN AEIKALRLSE RQREKACEEL TDELAKLDGK 

       130        140        150        160        170        180 
LKLTESLLQS KNLEIKKINE EKKASMAAQF AAEATLRRVH AAQKDDDMPP IEAILAPLEA 

       190        200        210        220        230        240 
ELKLARSEIG KLQEDNRALD RLTKSKEAAL LDAERTVETA LAKAALVDDL QNKNQELMKQ 

       250        260        270        280        290        300 
IEICQEENKI LDRMHRQKVA EVEKLTQTVR ELEEAVLAGG AAANAVRDYQ RKFQEMNEER 

       310        320        330        340        350        360 
KTLDRELARA KVTANRVATV VANEWKDGND KVMPVKQWLE ERRFLQGEMQ QLRDKLAISD 

       370        380        390        400        410        420 
RAAKSEAQLK DKFQLRLRVL EETLRGTSSI SIRNTPEGRS MSNGPSRRQS IGGSDNLQKF 

       430        440        450        460        470        480 
ASNGFLSKKT PMRNSFTSNS TSVLKNAKGT SKSFDGGTRS LDRGKALLKG PGNYSFNKAC 

       490        500        510        520        530        540 
DETKESESPN TWKEDSEEKP PSELPAPATE DNVPGVLYDL LQKEVVALRK SSHEKDQSLK 

       550        560        570        580        590        600 
DKDDAIEMLA KKVETLTKAM EVEAKKMRRE VAAMEKEVAA MRVDKDQDNR AKRSSNTKPS 

       610        620 
SNTAQILAAR AAGRSGLTRS TQ 

References and documentation are available.

Number of amino acids: 622

Molecular weight: 69290.24

Theoretical pI: 8.68

Amino acid composition: 
Ala (A)  70	 11.3%
Arg (R)  48	  7.7%
Asn (N)  29	  4.7%
Asp (D)  39	  6.3%
Cys (C)   3	  0.5%
Gln (Q)  27	  4.3%
Glu (E)  65	 10.5%
Gly (G)  25	  4.0%
His (H)   4	  0.6%
Ile (I)  17	  2.7%
Leu (L)  62	 10.0%
Lys (K)  61	  9.8%
Met (M)  15	  2.4%
Phe (F)  12	  1.9%
Pro (P)  22	  3.5%
Ser (S)  50	  8.0%
Thr (T)  33	  5.3%
Trp (W)   3	  0.5%
Tyr (Y)   5	  0.8%
Val (V)  32	  5.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 104
Total number of positively charged residues (Arg + Lys): 109

Atomic composition:

Carbon      C	      2966
Hydrogen    H	      4928
Nitrogen    N	       894
Oxygen      O	       975
Sulfur      S	        18

Formula: C2966H4928N894O975S18
Total number of atoms: 9781

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    24075
Abs 0.1% (=1 g/l)   0.347, assuming all pairs of Cys residues form cystines


Ext. coefficient    23950
Abs 0.1% (=1 g/l)   0.346, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 52.81
This classifies the protein as unstable.



Aliphatic index: 75.71

Grand average of hydropathicity (GRAVY): -0.807