ProtParam
User-provided sequence:
10 20 30 40 50 60
MSDYVELLKR GGNEAIKINP PTGADFHITS RGSDWLFTVF CVNLLFGVIL VPLMFRKPVK
70 80 90 100 110 120
DRFVYYTAIA PNLFMSIAYF TMASNLGWIP VRAKYNHVQT STQKEHPGYR QIFYARYVGW
130 140 150 160 170 180
FLAFPWPIIQ MSLLGGTPLW QIAFNVGMTE IFTVCWLIAA CVHSTYKWGY YTIGIGAAIV
190 200 210 220 230 240
VCISLMTTTF NLVKARGKDV SNVFITFMSV IMFLWLIAYP TCFGITDGGN VLQPDSATIF
250 260 270 280 290 300
YGIIDLLILS ILPVLFMPLA NYLGIERLGL IFDEEPAEHV GPVAEKKMPS PASFKSSDSD
310 320 330 340
SSIKEKLKLK KKHKKDKKKA KKAKKAKKAK KAQEEEEDVA TDSE
References and
documentation are available.
Number of amino acids: 344
Molecular weight: 38719.54
Theoretical pI: 9.25
Total number of negatively charged residues (Asp + Glu): 29
Total number of positively charged residues (Arg + Lys): 40
Atomic composition:
Carbon C 1800
Hydrogen H 2786
Nitrogen N 440
Oxygen O 476
Sulfur S 16
Formula: C
1800H
2786N
440O
476S
16
Total number of atoms: 5518
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 65110
Abs 0.1% (=1 g/l) 1.682, assuming all pairs of Cys residues form cystines
Ext. coefficient 64860
Abs 0.1% (=1 g/l) 1.675, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 44.44
This classifies the protein as unstable.
Aliphatic index: 94.97
Grand average of hydropathicity (GRAVY): 0.145