ProtParam
User-provided sequence:
10 20 30 40 50 60
MSEATLSSKQ TIEWENKYSA HNYHPLPVVF HKAKGAHVWD PEGKLYLDFL SAYSAVNQGH
70 80 90 100 110 120
CHPHIIKALT EQAQTLTLSS RAFHNDVYAQ FAKFVTEFFG FETVLPMNTG AEAVETALKL
130 140 150 160 170 180
ARRWGYMKKN IPQDKAIILG AEGNFHGRTF GAISLSTDYE DSKLHFGPFV PNVASGHSVH
190 200 210 220 230 240
KIRYGHAEDF VPILESPEGK NVAAIILEPI QGEAGIVVPP ADYFPKVSAL CRKHNVLLIV
250 260 270 280 290 300
DEIQTGIGRT GELLCYDHYK AEAKPDIVLL GKALSGGVLP VSCVLSSHDI MSCFTPGSHG
310 320 330 340 350 360
STFGGNPLAS RVAIAALEVI RDEKLCQRAA QLGSSFIAQL KALQAKSNGI ISEVRGMGLL
370 380 390 400 410 420
TAIVIDPSKA NGKTAWDLCL LMKDHGLLAK PTHDHIIRLA PPLVISEEDL QTGVETIAKC
IDLL
References and
documentation are available.
Number of amino acids: 424
Molecular weight: 46086.01
Theoretical pI: 6.46
Total number of negatively charged residues (Asp + Glu): 44
Total number of positively charged residues (Arg + Lys): 39
Atomic composition:
Carbon C 2078
Hydrogen H 3267
Nitrogen N 557
Oxygen O 599
Sulfur S 14
Formula: C
2078H
3267N
557O
599S
14
Total number of atoms: 6515
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 38890
Abs 0.1% (=1 g/l) 0.844, assuming all pairs of Cys residues form cystines
Ext. coefficient 38390
Abs 0.1% (=1 g/l) 0.833, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 38.15
This classifies the protein as stable.
Aliphatic index: 97.36
Grand average of hydropathicity (GRAVY): 0.010