ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSEDKAKLGT TRSATEYRLS IGSAPTSRRS SMGESSSLMK FADQEGLTSS VGEYNENTIQ 

        70         80         90        100        110        120 
QLLLPKIREL SDSIITLDSN FTRLNFIHES LADLNESLGS LLYGIMSNSW CVEFSQAPHD 

       130        140        150        160        170        180 
IQDDLIAIKQ LKSLEDEKNN LVMELSNMER GIKRKKDEQG ENDLAKASQN KQFNQPLFPS 

       190        200        210        220        230        240 
SQVRKYRSYD NRDKRKPSKI GNNLQVENEE DYEDDTSSEA SFVLNPTNIG MSKSSQGHVT 

       250        260        270        280        290        300 
KTTRLNNNTN SKLRRKSILH TIRNSIASGA DLPIENDNVV NLGDLHPNNR ISLGSGAARV 

       310        320        330        340 
VNGPVTKNRN SMFSGRAERK PTESRHSVAK KTEKKINTRP PFR 

References and documentation are available.

Number of amino acids: 343

Molecular weight: 38421.86

Theoretical pI: 9.20

Amino acid composition: 
Ala (A)  16	  4.7%
Arg (R)  24	  7.0%
Asn (N)  32	  9.3%
Asp (D)  19	  5.5%
Cys (C)   1	  0.3%
Gln (Q)  13	  3.8%
Glu (E)  25	  7.3%
Gly (G)  18	  5.2%
His (H)   6	  1.7%
Ile (I)  19	  5.5%
Leu (L)  31	  9.0%
Lys (K)  26	  7.6%
Met (M)   8	  2.3%
Phe (F)   9	  2.6%
Pro (P)  13	  3.8%
Ser (S)  44	 12.8%
Thr (T)  19	  5.5%
Trp (W)   1	  0.3%
Tyr (Y)   6	  1.7%
Val (V)  13	  3.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 44
Total number of positively charged residues (Arg + Lys): 50

Atomic composition:

Carbon      C	      1641
Hydrogen    H	      2677
Nitrogen    N	       499
Oxygen      O	       546
Sulfur      S	         9

Formula: C1641H2677N499O546S9
Total number of atoms: 5372

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    14440
Abs 0.1% (=1 g/l)   0.376, assuming all pairs of Cys residues form cystines


Ext. coefficient    14440
Abs 0.1% (=1 g/l)   0.376, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 44.49
This classifies the protein as unstable.



Aliphatic index: 72.51

Grand average of hydropathicity (GRAVY): -0.862