ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSEEFIAVST LARNLEIAKG NEFHTILATL RSPVYINEQL LKSELSFLVT KILKLIRSGN 

        70         80         90        100        110        120 
DFDLWKGCHT SVVTCAYNPL VLSTHGGQLL AAIYSRLEQK TGFYSSVISS SHGKQLFNTL 

       130        140        150        160        170        180 
ISSVAIIIDL MKNKPTLSRE ALVPKLKAII PTLITLSQYE PELVLPVLQR ILKRNTTTFK 

       190        200        210        220        230        240 
PFTNKFRTVL INLIISDYAS LGTKTQRLVC ENFAYLHLLK IQVSDTSDDE TQAHHKIYAD 

       250        260        270        280        290        300 
SNWRTGLMSI LSQFKPIIQL CGEILDFEQD NELYKLIKSL PVIDESNNKE EFLPSLKLDF 

       310        320        330        340        350        360 
NAPLTLWEIP QRLSLLADML VAFISLPTPF PIRVPLGGIN SLCEVLLGVS NKYLPLKKEL 

       370        380        390        400        410        420 
RHDNELNGVI NTILPQIQFQ GIRLWEIMVS KYGKCGLSFF EGILSSIELF IPLKKKSNNE 

       430        440        450        460        470        480 
IDFNVVGSLK FEFATVFRLV NMILSHLGHQ LNIISVISQL IEVALFLSHD KTLIDSLFKN 

       490        500        510        520        530        540 
RKSIMKQQTK TKQSKRSKSA EGAFSDIYTH PELFVCKNSM NWFNEINDFF ITALNNWILP 

       550        560        570        580        590        600 
STPHIQILKY SITQSLRLKE RFGYIPESFV NLLRCEVLHP GSERVSILPI AISLLKNIND 

       610        620        630        640        650        660 
DMFELLCHPK VPVGMVYQLH KPLDLGEDGE VRDDINKKEV ETNESSSNAN TGLETLKALE 

       670        680        690        700        710        720 
NLENVTIPEP KHEVPKVVDD TAIFKKRSVE EVIERESTSS HKKVKFVEET TVDNGEELIV 

       730        740        750        760 
KKAVSQTKEE EKPMEDSEDE EQEEFEIPAI ELSDDEEEEE EGE 

References and documentation are available.

Number of amino acids: 763

Molecular weight: 86666.95

Theoretical pI: 5.48

Amino acid composition: 
Ala (A)  28	  3.7%
Arg (R)  24	  3.1%
Asn (N)  43	  5.6%
Asp (D)  32	  4.2%
Cys (C)   9	  1.2%
Gln (Q)  26	  3.4%
Glu (E)  72	  9.4%
Gly (G)  29	  3.8%
His (H)  18	  2.4%
Ile (I)  66	  8.7%
Leu (L)  99	 13.0%
Lys (K)  59	  7.7%
Met (M)  11	  1.4%
Phe (F)  37	  4.8%
Pro (P)  34	  4.5%
Ser (S)  66	  8.7%
Thr (T)  42	  5.5%
Trp (W)   6	  0.8%
Tyr (Y)  15	  2.0%
Val (V)  47	  6.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 104
Total number of positively charged residues (Arg + Lys): 83

Atomic composition:

Carbon      C	      3915
Hydrogen    H	      6253
Nitrogen    N	      1005
Oxygen      O	      1164
Sulfur      S	        20

Formula: C3915H6253N1005O1164S20
Total number of atoms: 12357

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    55850
Abs 0.1% (=1 g/l)   0.644, assuming all pairs of Cys residues form cystines


Ext. coefficient    55350
Abs 0.1% (=1 g/l)   0.639, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 43.90
This classifies the protein as unstable.



Aliphatic index: 105.87

Grand average of hydropathicity (GRAVY): -0.139