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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSEEGPQVKI REASKDNVDF ILSNVDLAMA NSLRRVMIAE IPTLAIDSVE VETNTTVLAD

70 80 90 100 110 120
EFIAHRLGLI PLQSMDIEQL EYSRDCFCED HCDKCSVVLT LQAFGESEST TNVYSKDLVI

130 140 150 160 170 180
VSNLMGRNIG HPIIQDKEGN GVLICKLRKG QELKLTCVAK KGIAKEHAKW GPAAAIEFEY

190 200 210 220 230 240
DPWNKLKHTD YWYEQDSAKE WPQSKNCEYE DPPNEGDPFD YKAQADTFYM NVESVGSIPV

250 260 270 280 290 300
DQVVVRGIDT LQKKVASILL ALTQMDQDKV NFASGDNNTA SNMLGSNEDV MMTGAEQDPY

310
SNASQMGNTG SGGYDNAW


References and documentation are available.

Number of amino acids: 318 Molecular weight: 35297.55 Theoretical pI: 4.52
Amino acid composition: 
Ala (A) 24 7.5% Arg (R) 8 2.5% Asn (N) 20 6.3% Asp (D) 26 8.2% Cys (C) 7 2.2% Gln (Q) 15 4.7% Glu (E) 25 7.9% Gly (G) 20 6.3% His (H) 5 1.6% Ile (I) 18 5.7% Leu (L) 23 7.2% Lys (K) 20 6.3% Met (M) 11 3.5% Phe (F) 8 2.5% Pro (P) 12 3.8% Ser (S) 23 7.2% Thr (T) 15 4.7% Trp (W) 5 1.6% Tyr (Y) 10 3.1% Val (V) 23 7.2% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 51 Total number of positively charged residues (Arg + Lys): 28 Atomic composition: Carbon C 1537 Hydrogen H 2406 Nitrogen N 412 Oxygen O 504 Sulfur S 18 Formula: C1537H2406N412O504S18 Total number of atoms: 4877 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 42775 Abs 0.1% (=1 g/l) 1.212, assuming all pairs of Cys residues form cystines Ext. coefficient 42400 Abs 0.1% (=1 g/l) 1.201, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 33.70 This classifies the protein as stable. Aliphatic index: 78.81 Grand average of hydropathicity (GRAVY): -0.426