ProtParam
User-provided sequence:
10 20 30 40 50 60
MSEEGPQVKI REASKDNVDF ILSNVDLAMA NSLRRVMIAE IPTLAIDSVE VETNTTVLAD
70 80 90 100 110 120
EFIAHRLGLI PLQSMDIEQL EYSRDCFCED HCDKCSVVLT LQAFGESEST TNVYSKDLVI
130 140 150 160 170 180
VSNLMGRNIG HPIIQDKEGN GVLICKLRKG QELKLTCVAK KGIAKEHAKW GPAAAIEFEY
190 200 210 220 230 240
DPWNKLKHTD YWYEQDSAKE WPQSKNCEYE DPPNEGDPFD YKAQADTFYM NVESVGSIPV
250 260 270 280 290 300
DQVVVRGIDT LQKKVASILL ALTQMDQDKV NFASGDNNTA SNMLGSNEDV MMTGAEQDPY
310
SNASQMGNTG SGGYDNAW
References and
documentation are available.
Number of amino acids: 318
Molecular weight: 35297.55
Theoretical pI: 4.52
Total number of negatively charged residues (Asp + Glu): 51
Total number of positively charged residues (Arg + Lys): 28
Atomic composition:
Carbon C 1537
Hydrogen H 2406
Nitrogen N 412
Oxygen O 504
Sulfur S 18
Formula: C
1537H
2406N
412O
504S
18
Total number of atoms: 4877
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 42775
Abs 0.1% (=1 g/l) 1.212, assuming all pairs of Cys residues form cystines
Ext. coefficient 42400
Abs 0.1% (=1 g/l) 1.201, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 33.70
This classifies the protein as stable.
Aliphatic index: 78.81
Grand average of hydropathicity (GRAVY): -0.426