ProtParam
User-provided sequence:
10 20 30 40 50 60
MSEEHIAVTR FREYLRVNTE QPNPDYAACR DFLFKYADEL GIARRSFETV PGAIFVIMTI
70 80 90 100 110 120
PGSQPELPSI MLYSHTDVVP TFREHWTHDP YSAFKDEDGN IFARGAQDMK CVGVQQMEAL
130 140 150 160 170 180
RNLFAQGIRQ WKRTIHLVWG PDEEIFGING MKGFAKTDEF KKLNLGFSLD EGMPSDDDVY
190 200 210 220 230 240
KVFYAERVAW WVKVTFPGNP GHGSQFMENT AMEKLERYLA SARKFRDEQK ALLESNPDLT
250 260 270 280 290 300
IGDVTTLNVN IVNGGVQFNV IPEKFEAFVD IRLTPSIDFN EMRNKLDQWV KDAGEGVTYE
310 320 330 340 350 360
FSKHSDLKLV TPHTRDDPFW VAFEDSLKQE KCKFTTEVLI GSTDSRIVRE AGVRAINFSP
370 380 390
LINTPLLAHA HNEFLNEKVF LRGIEIYQTL INNVANVNE
References and
documentation are available.
Number of amino acids: 399
Molecular weight: 45679.73
Theoretical pI: 5.22
Total number of negatively charged residues (Asp + Glu): 59
Total number of positively charged residues (Arg + Lys): 44
Atomic composition:
Carbon C 2057
Hydrogen H 3148
Nitrogen N 550
Oxygen O 605
Sulfur S 13
Formula: C
2057H
3148N
550O
605S
13
Total number of atoms: 6373
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 53525
Abs 0.1% (=1 g/l) 1.172, assuming all pairs of Cys residues form cystines
Ext. coefficient 53400
Abs 0.1% (=1 g/l) 1.169, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 31.06
This classifies the protein as stable.
Aliphatic index: 79.15
Grand average of hydropathicity (GRAVY): -0.370