ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSEFLEDPSV LTKDKLKSAL LANNVALPNG DQRKDVYVQL YLKNLTVQNK KSSGSPDVFS 

        70         80         90        100        110        120 
SDEELPPAPV VSNRSRSGRK ATRKTDKVRP DDVDVTELSN EGLKDLLLKY GLNAGPIVAS 

       130        140        150        160        170        180 
TRKVYEKRLQ KLLDQGPPVA VALPSETSQT DGNQNGNNDS DQYSDREEEP VAPAPVTVPE 

       190        200        210        220        230        240 
PEVEAELIPV VERPVRSRGK TPVTSRTRSG QHTREDVEEE DWPVLNVKRK LKRSSHRPDQ 

       250        260        270        280        290        300 
MVPASDDTEN SELSSAECFA VSEDRRRTGP GAGRRETRPL LSDRTSKLSS KSESLRRRSA 

       310        320        330        340        350        360 
PVRSVLNEAS SSDKDFSEPS IVKEVSVSLM RMKVQPLTVP KDPKPSKRYS MSATESTKRP 

       370        380        390        400        410        420 
VSSLNNKHDE NTADIPSPPH RQSSREPLVS LINTACVEVD DQGMQDVLSC RSANGGLLAQ 

       430        440        450        460        470        480 
GVRSAAVSKL SKPVLSQSKP SKPLVDMMCC LSPSSDRQKE VFPSHSLPYK NFCLVSEDSL 

       490        500        510        520        530        540 
TVSCELSQES CGSPQTHPKS RHSKITPFLS QITPVRGLDN KLMDMKETMM VEKVSAIDQT 

       550        560        570        580        590        600 
PRAVERDVLK EIFPTENLNT PTGISATCRR PIRGAAGRPL IDTWLNESRQ RLSDLKQTTS 

       610        620        630        640        650        660 
SSSFSSTSSY TESRSVPRVS SIPLSASKST APPTVKSRSR RSLPVWVQLV LLSAVAGFLF 

       670        680 
FIYQAMETND VGLFKQSGTD DSTSK 

References and documentation are available.

Number of amino acids: 685

Molecular weight: 75328.81

Theoretical pI: 8.97

Amino acid composition: 
Ala (A)  35	  5.1%
Arg (R)  52	  7.6%
Asn (N)  26	  3.8%
Asp (D)  43	  6.3%
Cys (C)   9	  1.3%
Gln (Q)  26	  3.8%
Glu (E)  45	  6.6%
Gly (G)  26	  3.8%
His (H)   7	  1.0%
Ile (I)  14	  2.0%
Leu (L)  61	  8.9%
Lys (K)  46	  6.7%
Met (M)  13	  1.9%
Phe (F)  13	  1.9%
Pro (P)  55	  8.0%
Ser (S) 100	 14.6%
Thr (T)  42	  6.1%
Trp (W)   3	  0.4%
Tyr (Y)   9	  1.3%
Val (V)  60	  8.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 88
Total number of positively charged residues (Arg + Lys): 98

Atomic composition:

Carbon      C	      3234
Hydrogen    H	      5308
Nitrogen    N	       956
Oxygen      O	      1065
Sulfur      S	        22

Formula: C3234H5308N956O1065S22
Total number of atoms: 10585

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    30410
Abs 0.1% (=1 g/l)   0.404, assuming all pairs of Cys residues form cystines


Ext. coefficient    29910
Abs 0.1% (=1 g/l)   0.397, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 62.80
This classifies the protein as unstable.



Aliphatic index: 73.21

Grand average of hydropathicity (GRAVY): -0.664