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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSEFLNENPD ILEENQLPTR KEDSTKDLLL GGFSNEATLE RRSLLLKIDH SLKSQVLQDI

70 80 90 100 110 120
EVLDKLLSIR IPPELTSDED SLPAESEDES VAGGGKEEEE PDLIDAQEIY DLIAHISDPE

130 140 150 160 170 180
HPLSLGQLSV VNLEDIDVHD SGNQNEMAEV VIKITPTITH CSLATLIGLG IRVRLERSLP

190 200 210 220 230
PRFRITILLK KGTHDSENQV NKQLNDKERV AAACENEQLL GVVSKMLVTC K


References and documentation are available.

Number of amino acids: 231 Molecular weight: 25675.00 Theoretical pI: 4.56
Amino acid composition: 
Ala (A) 10 4.3% Arg (R) 10 4.3% Asn (N) 11 4.8% Asp (D) 18 7.8% Cys (C) 3 1.3% Gln (Q) 9 3.9% Glu (E) 27 11.7% Gly (G) 11 4.8% His (H) 6 2.6% Ile (I) 17 7.4% Leu (L) 34 14.7% Lys (K) 13 5.6% Met (M) 3 1.3% Phe (F) 3 1.3% Pro (P) 11 4.8% Ser (S) 19 8.2% Thr (T) 11 4.8% Trp (W) 0 0.0% Tyr (Y) 1 0.4% Val (V) 14 6.1% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 45 Total number of positively charged residues (Arg + Lys): 23 Atomic composition: Carbon C 1114 Hydrogen H 1834 Nitrogen N 306 Oxygen O 373 Sulfur S 6 Formula: C1114H1834N306O373S6 Total number of atoms: 3633 Extinction coefficients: This protein does not contain any Trp residues. Experience shows that this could result in more than 10% error in the computed extinction coefficient. Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 1615 Abs 0.1% (=1 g/l) 0.063, assuming all pairs of Cys residues form cystines Ext. coefficient 1490 Abs 0.1% (=1 g/l) 0.058, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 52.35 This classifies the protein as unstable. Aliphatic index: 108.01 Grand average of hydropathicity (GRAVY): -0.366