ProtParam
User-provided sequence:
10 20 30 40 50 60
MSEFLNENPD ILEENQLPTR KEDSTKDLLL GGFSNEATLE RRSLLLKIDH SLKSQVLQDI
70 80 90 100 110 120
EVLDKLLSIR IPPELTSDED SLPAESEDES VAGGGKEEEE PDLIDAQEIY DLIAHISDPE
130 140 150 160 170 180
HPLSLGQLSV VNLEDIDVHD SGNQNEMAEV VIKITPTITH CSLATLIGLG IRVRLERSLP
190 200 210 220 230
PRFRITILLK KGTHDSENQV NKQLNDKERV AAACENEQLL GVVSKMLVTC K
References and
documentation are available.
Number of amino acids: 231
Molecular weight: 25675.00
Theoretical pI: 4.56
Total number of negatively charged residues (Asp + Glu): 45
Total number of positively charged residues (Arg + Lys): 23
Atomic composition:
Carbon C 1114
Hydrogen H 1834
Nitrogen N 306
Oxygen O 373
Sulfur S 6
Formula: C
1114H
1834N
306O
373S
6
Total number of atoms: 3633
Extinction coefficients:
This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 1615
Abs 0.1% (=1 g/l) 0.063, assuming all pairs of Cys residues form cystines
Ext. coefficient 1490
Abs 0.1% (=1 g/l) 0.058, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 52.35
This classifies the protein as unstable.
Aliphatic index: 108.01
Grand average of hydropathicity (GRAVY): -0.366