ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSEFNETKFS NNGTFFETEE PIVETKSISV YTPLIYVFIL VVSLVMFASS YRKKQAKKIS 

        70         80         90        100        110        120 
EQPSIFDEND AHDLYFQIKE MSENEKIHEK VLKAALLNRG AESVRRSLKL KELAPQINLL 

       130        140        150        160        170        180 
YKNGSIGEDY WKRFETEVKL IELEFKDTLQ EAERLQPGWV QLFVMVCKEI CFNQALSRRY 

       190        200 
QSILKRKEVC IKEWELKINN DGRLVN 

References and documentation are available.

Number of amino acids: 206

Molecular weight: 24230.95

Theoretical pI: 6.77

Amino acid composition: 
Ala (A)   9	  4.4%
Arg (R)  10	  4.9%
Asn (N)  12	  5.8%
Asp (D)   6	  2.9%
Cys (C)   3	  1.5%
Gln (Q)   9	  4.4%
Glu (E)  25	 12.1%
Gly (G)   6	  2.9%
His (H)   2	  1.0%
Ile (I)  15	  7.3%
Leu (L)  21	 10.2%
Lys (K)  21	 10.2%
Met (M)   4	  1.9%
Phe (F)  12	  5.8%
Pro (P)   5	  2.4%
Ser (S)  15	  7.3%
Thr (T)   7	  3.4%
Trp (W)   3	  1.5%
Tyr (Y)   7	  3.4%
Val (V)  14	  6.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 31
Total number of positively charged residues (Arg + Lys): 31

Atomic composition:

Carbon      C	      1096
Hydrogen    H	      1733
Nitrogen    N	       285
Oxygen      O	       319
Sulfur      S	         7

Formula: C1096H1733N285O319S7
Total number of atoms: 3440

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    27055
Abs 0.1% (=1 g/l)   1.117, assuming all pairs of Cys residues form cystines


Ext. coefficient    26930
Abs 0.1% (=1 g/l)   1.111, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 41.52
This classifies the protein as unstable.



Aliphatic index: 92.23

Grand average of hydropathicity (GRAVY): -0.405