ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSEFNITETY LRFLEEDTEM TMPIAAIEAL VTLLRIKTPE TAAEMINTIK SSTEELIKSI 

        70         80         90        100        110        120 
PNSVSLRAGC DIFMRFVLRN LHLYGDWENC KQHLIENGQL FVSRAKKSRN KIAEIGVDFI 

       130        140        150        160        170        180 
ADDDIILVHG YSRAVFSLLN HAANKFIRFR CVVTESRPSK QGNQLYTLLE QKGIPVTLIV 

       190        200        210        220        230        240 
DSAVGAVIDK VDKVFVGAEG VAESGGIINL VGTYSVGVLA HNARKPFYVV TESHKFVRMF 

       250        260        270        280        290        300 
PLSSDDLPMA GPPLDFTRRT DDLEDALRGP TIDYTAQEYI TALITDLGVL TPSAVSEELI 


KMWYD 

References and documentation are available.

Number of amino acids: 305

Molecular weight: 34025.12

Theoretical pI: 5.18

Amino acid composition: 
Ala (A)  23	  7.5%
Arg (R)  16	  5.2%
Asn (N)  12	  3.9%
Asp (D)  19	  6.2%
Cys (C)   3	  1.0%
Gln (Q)   6	  2.0%
Glu (E)  22	  7.2%
Gly (G)  17	  5.6%
His (H)   6	  2.0%
Ile (I)  25	  8.2%
Leu (L)  30	  9.8%
Lys (K)  15	  4.9%
Met (M)   8	  2.6%
Phe (F)  14	  4.6%
Pro (P)  12	  3.9%
Ser (S)  20	  6.6%
Thr (T)  21	  6.9%
Trp (W)   2	  0.7%
Tyr (Y)   9	  3.0%
Val (V)  25	  8.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 41
Total number of positively charged residues (Arg + Lys): 31

Atomic composition:

Carbon      C	      1526
Hydrogen    H	      2426
Nitrogen    N	       400
Oxygen      O	       456
Sulfur      S	        11

Formula: C1526H2426N400O456S11
Total number of atoms: 4819

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    24535
Abs 0.1% (=1 g/l)   0.721, assuming all pairs of Cys residues form cystines


Ext. coefficient    24410
Abs 0.1% (=1 g/l)   0.717, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 41.28
This classifies the protein as unstable.



Aliphatic index: 101.64

Grand average of hydropathicity (GRAVY): 0.028