ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSEGFSSSSI QELYQSLKEI TNNADVELFE DRITKLDFES TDEPKHANDI IKDRFLRPSN 

        70         80         90        100        110        120 
ALPWSLLDMV QDVPHTSSPE DCSGKLDYKE LLKVPDPINR TSYQFKRTGL EGKISGYKEE 

       130        140        150        160        170        180 
VDLKEVANAN ASNSLSITRS INHNQNSVRG STAQLPFTPG GIPMKSVKTD SEQNGSSTMA 

       190        200        210        220        230        240 
NATKLLHKDG QGLFDIPEGM NRGIKPMDSP AENEDQNGQF KELKQLNEID NELDIRIEAN 

       250        260        270        280        290        300 
EAKLKEEEKS AKSISEEIME EATEETTADN ADDAEIDELL PIGIDFGRTK PVSKSVPVKK 

       310        320        330        340        350        360 
EWAHVVDLNH KIENFDELIP NPARSWPFEL DTFQKEAVYH LEQGDSVFVA AHTSAGKTVV 

       370        380        390        400        410        420 
AEYAIAMAHR NMTKTIYTSP IKALSNQKFR DFKETFDDVN IGLITGDVQI NPDANCLIMT 

       430        440        450        460        470        480 
TEILRSMLYR GADLIRDVEF VIFDEVHYVN DQDRGVVWEE VIIMLPQHVK FILLSATVPN 

       490        500        510        520        530        540 
TYEFANWIGR TKQKNIYVIS TPKRPVPLEI NIWAKKELIP VINQNSEFLE ANFRKHKEIL 

       550        560        570        580        590        600 
NGESAKGAPS KTDNGRGGST ARGGRGGSNT RDGRGGRGNS TRGGANRGGS RGAGAIGSNK 

       610        620        630        640        650        660 
RKFFTQDGPS KKTWPEIVNY LRKRELLPMV VFVFSKKRCE EYADWLEGIN FCNNKEKSQI 

       670        680        690        700        710        720 
HMFIEKSITR LKKEDRDLPQ ILKTRSLLER GIAVHHGGLL PIVKELIEIL FSKGFIKVLF 

       730        740        750        760        770        780 
ATETFAMGLN LPTRTVIFSS IRKHDGNGLR ELTPGEFTQM AGRAGRRGLD STGTVIVMAY 

       790        800        810        820        830        840 
NSPLSIATFK EVTMGVPTRL QSQFRLTYNM ILNLLRIEAL RVEEMIKYSF SENAKETLQP 

       850        860        870        880        890        900 
EHEKQIKVLQ EELQTIEYKS CEICDNDIEK FLELMLAYKE ATVNLMQEMV KSPSILHILK 

       910        920        930        940        950        960 
EGRLVAFRDP NDCLKLGFVF KVSLKDAVCV IMTFTKPYKL PNGEPNHLIY FPKADGYRRR 

       970        980        990       1000       1010       1020 
NFPKFQKTDF YMEEVPVTAI EVITKRKFAA PLGKVIKKDV AALNEFNAET NNILDGKTLK 

      1030       1040       1050       1060       1070       1080 
EAINIEKQGL KIHQILLDRT NIRDEIFKLK SIKCPNLSQH IVPKFKAHVI KKKIEELYHL 

      1090       1100       1110       1120       1130       1140 
MSDQNLSLLP DYEKRLAVLK DTEFIDQNHN VLLKGRVACE INSGYELVLT ELILDNFLGS 

      1150       1160       1170       1180       1190       1200 
FEPEEIVALL SVFVYEGKTR EEEPPIVTPR LAKGKQRIEE IYKKMLCVFN THQIPLTQDE 

      1210       1220       1230       1240       1250       1260 
AEFLDRKRFA MMNVVYEWAR GLSFKEIMEM SPEAEGTVVR VITWLDEICR EVKTASIIIG 

      1270       1280 
NSTLHMKMSR AQELIKRDIV FAASLYL 

References and documentation are available.

Number of amino acids: 1287

Molecular weight: 146058.51

Theoretical pI: 6.31

Amino acid composition: 
Ala (A)  75	  5.8%
Arg (R)  67	  5.2%
Asn (N)  73	  5.7%
Asp (D)  66	  5.1%
Cys (C)  12	  0.9%
Gln (Q)  40	  3.1%
Glu (E) 119	  9.2%
Gly (G)  73	  5.7%
His (H)  26	  2.0%
Ile (I) 100	  7.8%
Leu (L) 117	  9.1%
Lys (K) 108	  8.4%
Met (M)  33	  2.6%
Phe (F)  59	  4.6%
Pro (P)  56	  4.4%
Ser (S)  77	  6.0%
Thr (T)  71	  5.5%
Trp (W)  10	  0.8%
Tyr (Y)  29	  2.3%
Val (V)  76	  5.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 185
Total number of positively charged residues (Arg + Lys): 175

Atomic composition:

Carbon      C	      6508
Hydrogen    H	     10393
Nitrogen    N	      1771
Oxygen      O	      1948
Sulfur      S	        45

Formula: C6508H10393N1771O1948S45
Total number of atoms: 20665

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    98960
Abs 0.1% (=1 g/l)   0.678, assuming all pairs of Cys residues form cystines


Ext. coefficient    98210
Abs 0.1% (=1 g/l)   0.672, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 42.45
This classifies the protein as unstable.



Aliphatic index: 88.71

Grand average of hydropathicity (GRAVY): -0.403