ProtParam
User-provided sequence:
10 20 30 40 50 60
MSEGRTFLSQ LNVFNKENYQ FSSSTTKKEV SNSTVDADNG ASDFEAGQQF ATELDQGEKQ
70 80 90 100 110 120
LGILSCIGLI CNRMLGTGVF AVSSTIYTLC GSVGLALIMW AVGAIIAISG LYVYMEFGTA
130 140 150 160 170 180
IPKNGGEKNY LEAIFRKPKF FITCMYAAYI FFLGWAAGNS INTAIMFLTA ADTEVTKWNQ
190 200 210 220 230 240
RGIGVAVVFF AFLINSLNVK IGLYLQNILG IFKIGIVLFI SITGWVALGG GLKDGYQSHN
250 260 270 280 290 300
FRNAFEGTET ATAYGIVNAL YSVIWSFVGY SNVNYALGEV KNPVRTLKIA GPTSMVFLAI
310 320 330 340 350 360
IYIFVNIAYF AVVPKDKLIS SKLILAADFF DIVFGGQAKR AAAALVGLSA LGNVLSVIFS
370 380 390 400 410 420
QGRIIQQLGR EGVLPFSNFF ASSKPFNSPM VGLFQHFIVC TVTILAPPPG DAYLLVQNLI
430 440 450 460 470 480
SYPMNIINFA ISAGLLWIYW QRRQGKIEWN PPIKAGVFVT GFFTLSNLYL IIAPYVPPSN
490 500 510 520 530 540
GESVYSSMPY WIHCVIAWGI FFFGGVYYVV WAQLLPRWGH YKLVSKDVLG EDGFWRVKIA
550 560 570
KVYDDTIGDV DTQEDGVIET NIIEHYKSEQ EKSL
References and
documentation are available.
Number of amino acids: 574
Molecular weight: 63221.31
Theoretical pI: 7.54
Total number of negatively charged residues (Asp + Glu): 39
Total number of positively charged residues (Arg + Lys): 40
Atomic composition:
Carbon C 2949
Hydrogen H 4484
Nitrogen N 714
Oxygen O 798
Sulfur S 16
Formula: C
2949H
4484N
714O
798S
16
Total number of atoms: 8961
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 112105
Abs 0.1% (=1 g/l) 1.773, assuming all pairs of Cys residues form cystines
Ext. coefficient 111730
Abs 0.1% (=1 g/l) 1.767, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 29.24
This classifies the protein as stable.
Aliphatic index: 103.95
Grand average of hydropathicity (GRAVY): 0.405