ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSEKETNYVE NLLTQLENEL NEDNLPEDIN TLLRKCSLNL VTVVSLPDMD VKPLLATIKR 

        70         80         90        100        110        120 
FLTSNVSYDS LNYDYLLDVV DKLVPMADFD DVLEVYSAED LVKALRSEID PLKVAACRVI 

       130        140        150        160        170        180 
ENSQPKGLFA TSNIIDILLD ILFDEKVEND KLITAIEKAL ERLSTDELIR RRLFDNNLPY 

       190        200        210        220        230        240 
LVSVKGRMET VSFVRLIDFL TIEFQFISGP EFKDIIFCFT KEEILKSVED ILVFIELVNY 

       250        260        270        280        290        300 
YTKFLLEIRN QDKYWALRHV KKILPVFAQL FEDTENYPDV RAFSTNCLLQ LFAEVSRIEE 

       310        320        330        340        350        360 
DEYSLFKTMD KDSLKIGSEA KLITEWLELI NPQYLVKYHK DVVENYFHVS GYSIGMLRNL 

       370        380        390        400        410        420 
SADEECFNAI RNKFSAEIVL RLPYLEQMQV VETLTRYEYT SKFLLNEMPK VMGSLIGDGS 

       430        440        450        460        470        480 
AGAIIDLETV HYRNSALRNL LDKGEEKLSV WYEPLLREYS KAVNGKNYST GSETKIADCR 

References and documentation are available.

Number of amino acids: 480

Molecular weight: 55542.76

Theoretical pI: 4.73

Amino acid composition: 
Ala (A)  22	  4.6%
Arg (R)  22	  4.6%
Asn (N)  28	  5.8%
Asp (D)  34	  7.1%
Cys (C)   6	  1.2%
Gln (Q)   9	  1.9%
Glu (E)  47	  9.8%
Gly (G)  13	  2.7%
His (H)   4	  0.8%
Ile (I)  32	  6.7%
Leu (L)  67	 14.0%
Lys (K)  33	  6.9%
Met (M)   9	  1.9%
Phe (F)  23	  4.8%
Pro (P)  14	  2.9%
Ser (S)  32	  6.7%
Thr (T)  23	  4.8%
Trp (W)   3	  0.6%
Tyr (Y)  22	  4.6%
Val (V)  37	  7.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 81
Total number of positively charged residues (Arg + Lys): 55

Atomic composition:

Carbon      C	      2512
Hydrogen    H	      3965
Nitrogen    N	       627
Oxygen      O	       757
Sulfur      S	        15

Formula: C2512H3965N627O757S15
Total number of atoms: 7876

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    49655
Abs 0.1% (=1 g/l)   0.894, assuming all pairs of Cys residues form cystines


Ext. coefficient    49280
Abs 0.1% (=1 g/l)   0.887, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 39.43
This classifies the protein as stable.



Aliphatic index: 107.38

Grand average of hydropathicity (GRAVY): -0.133