ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSEKFPPLED QNIDFTPNDK KDDDTDFLKR EAEILGDEFK TEQDDILETE ASPAKDDDEI 

        70         80         90        100        110        120 
RDFEEQFPDI NSANGAVSSD QNGSATVSSG NDNGEADDDF STFEGANQST ESVKEDRSEV 

       130        140        150        160        170        180 
VDQWKQRRAV EIHEKDLKDE ELKKELQDEA IKHIDDFYDS YNKKKEQQLE DAAKEAEAFL 

       190        200        210        220        230 
KKRDEFFGQD NTTWDRALQL INQDDADIIG GRDRSKLKEI LLRLKGNAKA PGA 

References and documentation are available.

Number of amino acids: 233

Molecular weight: 26531.54

Theoretical pI: 4.31

Amino acid composition: 
Ala (A)  19	  8.2%
Arg (R)  10	  4.3%
Asn (N)  12	  5.2%
Asp (D)  36	 15.5%
Cys (C)   0	  0.0%
Gln (Q)  13	  5.6%
Glu (E)  28	 12.0%
Gly (G)  11	  4.7%
His (H)   2	  0.9%
Ile (I)  12	  5.2%
Leu (L)  15	  6.4%
Lys (K)  23	  9.9%
Met (M)   1	  0.4%
Phe (F)  12	  5.2%
Pro (P)   6	  2.6%
Ser (S)  14	  6.0%
Thr (T)   9	  3.9%
Trp (W)   2	  0.9%
Tyr (Y)   2	  0.9%
Val (V)   6	  2.6%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 64
Total number of positively charged residues (Arg + Lys): 33

Atomic composition:

Carbon      C	      1139
Hydrogen    H	      1773
Nitrogen    N	       317
Oxygen      O	       412
Sulfur      S	         1

Formula: C1139H1773N317O412S1
Total number of atoms: 3642

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    13980
Abs 0.1% (=1 g/l)   0.527

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 51.92
This classifies the protein as unstable.



Aliphatic index: 60.82

Grand average of hydropathicity (GRAVY): -1.213