ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSELFGTLSD GTPVHRWTLE RAGVRVRVLS YGGIVQSAEV PDRDGHTADV VLGFADLDGY 

        70         80         90        100        110        120 
VAHPEPYFGA LVGRYANRIA GGRFPLDGRT YALAPNEGPN TLHGGTRGFD KRVWDVAAVE 

       130        140        150        160        170        180 
EGVRLSRVSP HGEEGFPGRL EMSVTYTLDG SGALRIAYEA VTDAPTVLNP TNHSYFNLSG 

       190        200        210        220        230        240 
SGHAGGHELR LAASRITPVD AGLIPTGGLD DVTDTRFDFR RARKVGSGYD HNYVLDKGVT 

       250        260        270        280        290        300 
EAAEKVAELY DPASGRVLTV ATTEPGLQLY TADHLGEPFA PGDGIALETQ HFPDSPNRPG 

       310        320 
FPSTVLRPGE VFRSETVYGF SVR 

References and documentation are available.

Number of amino acids: 323

Molecular weight: 34680.50

Theoretical pI: 5.37

Amino acid composition: 
Ala (A)  29	  9.0%
Arg (R)  26	  8.0%
Asn (N)   8	  2.5%
Asp (D)  22	  6.8%
Cys (C)   0	  0.0%
Gln (Q)   3	  0.9%
Glu (E)  20	  6.2%
Gly (G)  41	 12.7%
His (H)  11	  3.4%
Ile (I)   6	  1.9%
Leu (L)  28	  8.7%
Lys (K)   4	  1.2%
Met (M)   2	  0.6%
Phe (F)  14	  4.3%
Pro (P)  22	  6.8%
Ser (S)  18	  5.6%
Thr (T)  24	  7.4%
Trp (W)   2	  0.6%
Tyr (Y)  13	  4.0%
Val (V)  30	  9.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 42
Total number of positively charged residues (Arg + Lys): 30

Atomic composition:

Carbon      C	      1539
Hydrogen    H	      2366
Nitrogen    N	       440
Oxygen      O	       474
Sulfur      S	         2

Formula: C1539H2366N440O474S2
Total number of atoms: 4821

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    30370
Abs 0.1% (=1 g/l)   0.876

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 34.98
This classifies the protein as stable.



Aliphatic index: 76.97

Grand average of hydropathicity (GRAVY): -0.310